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dc.contributor.authorHornemann, Jennifer A.
dc.contributor.authorLysova, Anna A.
dc.contributor.authorCodd, Sarah L.
dc.contributor.authorSeymour, Joseph D.
dc.contributor.authorBusse, S.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorBrown, Jennifer R.
dc.identifier.citationHornemann JA, Lysova AA, Codd SL, Seymour JD, Busse SC, Stewart PS, Brown JR, "Biopolymer and water dynamics in microbial biofilm extracellular polymeric substance," Biomacromolecules 2008; 9(9):2322-2328en_US
dc.description.abstractNuclear magnetic resonance (NMR) is a noninvasive and nondestructive tool able to access several observable quantities in biofilms such as chemical composition, diffusion, and macroscale structure and transport. Pulsed gradient spin echo (PGSE) NMR techniques were used to measure spectrally resolved biomacromolecular diffusion in biofilm biomass, extending previous research on spectrally resolved diffusion in biofilms. The dominant free water signal was nulled using an inversion recovery modification of the traditional PGSE technique in which the signal from free water is minimized in order to view the spectra of components such as the rotationally mobile carbohydrates, DNA, and proteins. Diffusion data for the major constituents obtained from each of these spectral peaks demonstrate that the biomass of the biofilm contains both a fast and slow diffusion component. The dependence of diffusion on antimicrobial and environmental challenges suggests the polymer molecular dynamics measured by NMR are a sensitive indicator of biofilm function.en_US
dc.titleBiopolymer and water dynamics in microbial biofilm extracellular polymeric substanceen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMechanical & Industrial Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US

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