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dc.contributor.authorBerra, Lorenzo
dc.contributor.authorCurto, Francesco
dc.contributor.authorBassi, Gianluigi L.
dc.contributor.authorLaquerriere, Patrice
dc.contributor.authorPitts, Betsey
dc.contributor.authorBaccarelli, Andrea
dc.contributor.authorKolobow, Theodor
dc.date.accessioned2017-07-07T18:56:07Z
dc.date.available2017-07-07T18:56:07Z
dc.date.issued2008-04
dc.identifier.citationBerra L, Curto F, Li Bassi G, Laquerriere P, Pitts B, Baccarelli A, Kolobow T, "Antimicrobial-coated endotracheal tubes: An experimental study," Intensive Care Med. 2008 34(6):1020-1029en_US
dc.identifier.issn0342-4642
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13207
dc.description.abstractOBJECTIVE: Antibiotic-resistant bacterial biofilm may quickly form on endotracheal tubes (ETTs) and can enter the lungs, potentially causing pneumonia. In an attempt to prevent bacterial colonization, we developed and tested in an in-vitro study and animal study several antibacterial-coated ETTs (silver sulfadiazine with and without carbon in polyurethane, silver sulfadiazine and chlorhexidine with and without carbon in polyurethane, silver-platinum with and without carbon in polyurethane, chlorhexidine in polyurethane, and rose bengal for UV light). DESIGN, SETTING, ANIMALS, INTERVENTIONS: After preliminary studies, silver sulfadiazine in polyurethane (SSD-ETT) was selected among the coatings to be challenged every 24h with 104-106 Pseudomonas aeruginosa/ml and evaluated at 6h, 24h, and 72h with standard microbiological studies, scanning electron microscopy, and confocal scanning microscopy. Subsequently, eight sheep were randomized to receive either a SSD-ETT or a standard ETT (St-ETT). After 24h of mechanical ventilation, standard microbiological studies were performed together with scanning electron microscopy and confocal microscopy. MEASUREMENTS AND RESULTS: In the in-vitro study SSD-ETT remained bacteria-free for up to 72h, whereas St-ETT showed heavy P. aeruginosa growth and biofilm formation (p < 0.01). In sheep, the SSD-ETT group showed no bacterial growth in the ETT, ventilator tubing, and lower respiratory tract, while heavy colonization was found in the St-ETT (p < 0.01), ventilator tubing (p = 0.03), and lower respiratory tract (p < 0.01). CONCLUSION: This study describes several effective and durable antibacterial coatings for ETTs. Particularly, SSD-ETT showed prevention against P. aeruginosa biofilm formation in a 72-h in-vitro study and lower respiratory tract colonization in sheep mechanically ventilated for 24h.en_US
dc.titleAntimicrobial-coated endotracheal tubes: An experimental studyen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1020en_US
mus.citation.extentlastpage1029en_US
mus.citation.issue6en_US
mus.citation.journaltitleIntensive Care Medicineen_US
mus.citation.volume34en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s00134-008-1099-3en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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