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dc.contributor.authorJames, Garth A.
dc.contributor.authorSwogger, E.
dc.contributor.authorWolcott, Randall D.
dc.contributor.authorPulcini, Elinor D.
dc.contributor.authorSecor, Patrick R.
dc.contributor.authorSestrich, Jennifer
dc.contributor.authorCosterton, J. William
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-07-12T13:37:31Z
dc.date.available2017-07-12T13:37:31Z
dc.date.issued2008-01
dc.identifier.citationJames GA, Swogger E, Wolcott R, deLancey Pulcini E, Secor P, Sestrich J, Costerton JW, Stewart PS, "Biofilms in chronic wounds," Wound Repair and Regeneration 2008 16:37-44.en_US
dc.identifier.issn1067-1927
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13213
dc.description.abstractChronic wounds including diabetic foot ulcers, pressure ulcers, and venous leg ulcers are a worldwide health problem. It has been speculated that bacteria colonizing chronic wounds exist as highly persistent biofilm communities. This research examined chronic and acute wounds for biofilms and characterized microorganisms inhabiting these wounds. Chronic wound specimens were obtained from 77 subjects and acute wound specimens were obtained from 16 subjects. Culture data were collected using standard clinical techniques. Light and scanning electron microscopy techniques were used to analyze 50 of the chronic wound specimens and the 16 acute wound specimens. Molecular analyses were performed on the remaining 27 chronic wound specimens using denaturing gradient gel electrophoresis and sequence analysis. Of the 50 chronic wound specimens evaluated by microscopy, 30 were characterized as containing biofilm (60%), whereas only one of the 16 acute wound specimens was characterized as containing biofilm (6%). This was a statistically significant difference (p<0.001). Molecular analyses of chronic wound specimens revealed diverse polymicrobial communities and the presence of bacteria, including strictly anaerobic bacteria, not revealed by culture. Bacterial biofilm prevalence in specimens from chronic wounds relative to acute wounds observed in this study provides evidence that biofilms may be abundant in chronic wounds.en_US
dc.titleBiofilms in chronic woundsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage37en_US
mus.citation.extentlastpage44en_US
mus.citation.issue1en_US
mus.citation.journaltitleWound Repair and Regenerationen_US
mus.citation.volume16en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1111/j.1524-475X.2007.00321.xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage42en_US
mus.contributor.orcidSecor, Patrick R.|0000-0001-7123-3037en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US


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