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dc.contributor.authorGoeres, Darla M.
dc.contributor.authorHamilton, Martin A.
dc.contributor.authorBeck, Nicholas A.
dc.contributor.authorBuckingham-Meyer, Kelli
dc.contributor.authorHilyard, Jackie D.
dc.contributor.authorLoetterle, Linda R.
dc.contributor.authorLorenz, Lindsey A.
dc.contributor.authorWalker, Diane K.
dc.contributor.authorStewart, Philip S.
dc.identifier.citationGoeres DM, Hamilton MA, Beck NA, Buckingham-Meyer K, Hilyard JD, Loetterle LR, Lorenz LA, Walker DK, Stewart PS, "A method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactor," Nature Protocols 2009 4(5):783-788en_US
dc.description.abstractThis protocol describes how to grow a Pseudomonas aeruginosa biofilm under low fluid shear close to the air–liquid interface using the drip flow reactor (DFR). The DFR can model environments such as food-processing conveyor belts, catheters, lungs with cystic fibrosis and the oral cavity. The biofilm is established by operating the reactor in batch mode for 6 h. A mature biofilm forms as the reactor operates for an additional 48 h with a continuous flow of nutrients. During continuous flow, the biofilm experiences a low shear as the media drips onto a surface set at a 101 angle. At the end of 54 h, biofilm accumulation is quantified by removing coupons from the reactor channels, rinsing the coupons to remove planktonic cells, scraping the biofilm from the coupon surface, disaggregating the clumps, then diluting and plating for viable cell enumeration. The entire procedure takes 13 h of active time that is distributed over 5 d.en_US
dc.titleA method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactoren_US
mus.citation.journaltitleNature Protocolsen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US

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