Show simple item record

dc.contributor.authorAltman, S. J.
dc.contributor.authorMcGrath, L. K.
dc.contributor.authorSouza, C. A.
dc.contributor.authorMurton, J. K.
dc.contributor.authorCamper, Anne K.
dc.date.accessioned2017-07-13T18:00:34Z
dc.date.available2017-07-13T18:00:34Z
dc.date.issued2009-07
dc.identifier.citationAltman SJ, McGrath LK, Souza SA, Murton JK, Camper AK, "Integration and decontamination of Bacillus cereus in Pseudomonas fluorescens biofilms," Journal of Applied Microbiology 2009 107, 287–299en_US
dc.identifier.issn1364-5072
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13247
dc.description.abstractAims:  The hypothesis that surrogate planktonic pathogens (Bacillus cereus and polystyrene microspheres) could be integrated in biofilms and protected from decontamination was tested. Methods and Results: Pseudomonas fluorescens biofilms were grown on polyvinyl chloride coupons in annular reactors under low nutrient conditions. After biofilm growth, B. cereus spores and polystyrene microspheres (an abiotic control) were introduced separately. Shear stress at the biofilm surface was varied between 0·15 and 1·5 N m−2. The amount of surrogate pathogens introduced ranged from approximately 105 CFU ml−1 to 1010 spheres ml−1. The quantity of surrogate pathogens integrated in the biofilm was proportional to the amount introduced. In 14 of the 16 cases, 0·4–3·0% of the spores or spheres introduced were measured in the biofilms. The other two cases had 10% and 21% of the spores detected. Data suggested that the spores germinated in the system. The amount of surrogate pathogens detected in the biofilms was higher in the mid-shear range. Chlorine treatment reduced the quantity of both surrogate pathogens and biofilm organisms. In one experiment, the biofilms and B. cereus recovered when the chlorine treatment was terminated. Conclusions:  Planktonic surrogate pathogens can be integrated in biofilms and protected from chlorination decontamination. Significance and Impact of the Study:  This knowledge assists in understanding the impact of biofilms on harbouring potential pathogens in drinking-water systems and protecting the pathogens from decontamination.en_US
dc.titleIntegration and decontamination of Bacillus cereus in Pseudomonas fluorescens biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage287en_US
mus.citation.extentlastpage299en_US
mus.citation.issue1en_US
mus.citation.journaltitleJournal of Applied Microbiologyen_US
mus.citation.volume107en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1111/j.1365-2672.2009.04206.xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage7en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record