Show simple item record

dc.contributor.authorZiganshin, Ayrat M.
dc.contributor.authorGerlach, Robin
dc.contributor.authorBorch, Thomas
dc.contributor.authorNaumov, A. V.
dc.contributor.authorNaumova, R. P.
dc.date.accessioned2017-07-13T18:30:28Z
dc.date.available2017-07-13T18:30:28Z
dc.date.issued2007-10
dc.identifier.citationZiganshin AM, Gerlach R, Borch T, Naumov AV, Naumova RP, "Production of eight different hydride complexes and nitrite release from 2,4,6-trinitrotoluene by Yarrowia lipolytica," Appl Environ Microbiol 2007 73(24):7898-7905en_US
dc.identifier.issn0099-2240
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13261
dc.description.abstract2,4,6-Trinitrotoluene (TNT) transformation by the yeast strain Yarrowia lipolytica AN-L15 was shown to occur via two different pathways. Direct aromatic ring reduction was the predominant mechanism of TNT transformation, while nitro group reduction was observed to be a minor pathway. Although growth of Y.lipolytica AN-L15 was inhibited initially in the presence of TNT, TNT transformation was observed, indicating that the enzymes necessary for TNT reduction were present initially. Aromatic ring reduction resulted in the transient accumulation of eight different TNT-hydride complexes, which were characterized using high performance liquid chromatography, UV-visible diode array detection, and negative-mode atmospheric pressure chemical ionization mass spectrometry (APCI-MS). APCI-MS analysis revealed three different groups of TNT-hydride complexes with molecular ions at m/z 227, 228, and 230, which correspond to TNT-mono- and dihydride complexes and protonated dihydride isomers, respectively. One of the three protonated dihydride complex isomers detected appears to release nitrite in the presence of strain AN-L15. This release of nitrite is of particular interest since it can provide a pathway towards complete degradation and detoxification of TNT.en_US
dc.titleProduction of eight different hydride complexes and nitrite release from 2,4,6-trinitrotoluene by Yarrowia lipolyticaen_US
dc.typeArticleen_US
mus.citation.extentfirstpage7898en_US
mus.citation.extentlastpage7905en_US
mus.citation.issue24en_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.citation.volume73en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aem.01296-07en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.