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dc.contributor.authorKomlos, John
dc.contributor.authorCunningham, Alfred B.
dc.contributor.authorCamper, Anne K.
dc.contributor.authorSharp, Robert R.
dc.date.accessioned2017-07-13T22:29:15Z
dc.date.available2017-07-13T22:29:15Z
dc.date.issued2006
dc.identifier.citationKomlos J, Cunningham AB, Camper AK, Sharp RR, "Effect of substrate concentration on dual-species biofilm population densities of Klebsiella oxytoca and Burkholderia cepacia in porous media," Biotechnol Bioeng 2006 93(3):434-442en_US
dc.identifier.issn0006-3592
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13299
dc.description.abstractThe long-term operation of bioremediation technologies relies on the success of the contaminant-degrading microorganism(s) to compete for available resources with microorganisms already present in an aquifer or those that may contaminate a bioreactor. Though research has been performed studying the interaction of multiple species in batch and chemostat reactors, little work has been done looking at multi-species interactions in environments that more closely resemble field-scale applications. The research presented herein examined the interaction of Burkholderia cepacia PR1-pTOM31c, an aerobic trichloroethylene (TCE)-degrading bacterium, with Klebsiella oxytoca, a facultative bacterium, in a flow-through porous media (PM) reactor. Growth characteristics and population distributions in PM were compared to previously reported values from batch and chemostat reactors. The faster growing organism in batch experiments (K. oxytoca) did not always have the greater population density in dual-species PM experiments. The biofilm population distribution was influenced by substrate concentration, with B. cepacia having a greater dual-species population density than K. oxytoca at a low (30 mg/L dissolved organic carbon [DOC]) substrate concentration and K. oxytoca having a greater population density at a high (700 mg/L DOC) substrate concentration. This change in species population distribution with change in substrate concentration, which was not observed in batch reactors, was also observed in chemostat reactors. Therefore, manipulation of substrate concentration enabled the control of species dominance to the advantage of the TCE degrading population in this dual-species PM system and may provide a mechanism to enhance bioremediation scenarios involving TCE or other contaminants of concern. © 2005 Wiley Periodicals, Inc.en_US
dc.titleEffect of substrate concentration on dual-species biofilm population densities of Klebsiella oxytoca and Burkholderia cepacia in porous mediaen_US
dc.typeArticleen_US
mus.citation.extentfirstpage434en_US
mus.citation.extentlastpage442en_US
mus.citation.issue3en_US
mus.citation.journaltitleBiotechnology and Bioengineeringen_US
mus.citation.volume93en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1002/bit.20727en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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