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dc.contributor.authorPatrauchan, Marianna A.
dc.contributor.authorSarkisova, S. A.
dc.contributor.authorSauer, K.
dc.contributor.authorFranklin, Michael J.
dc.identifier.citationPatrauchan MA, Sarkisova S, Sauer K, Franklin MJ, "Calcium influences cellular and extracellular product formation during biofilm-associated growth of a marine Pseudoalteromonas sp.," Microbiology, 2005 151(9):2885-2897en_US
dc.description.abstractBacteria undergo a variety of physiological changes following a switch from planktonic growth to surface-associated biofilm growth. Here, it is shown that biofilm development of a marine isolate, Pseudoalteromonas sp. 1398, results in global changes in its cytosolic and extracellular proteomes. Calcium influences these proteome responses, and affects the amount of surface-associated biomass and extracellular matrix material produced by Pseudoalteromonassp. 1398. Four extracellular proteins, characterized by N-terminalsequencing, showed increased abundances, while one protein, flagellin, showed reduced abundance at higher [Ca2+]. Immunoblottingand transmission-electron-microscopy analysis confirmed that higher [Ca2+] and surface-associated growth results in the repressionof flagella production. Two-dimensional gel electrophoresis (2DGE) studies combined with cluster analysis of global proteome responses demonstrated that Ca2+ had a greater regulatory influenceon Pseudoalteromonas sp. growing in biofilms than on planktoniccultures. Approximately 22% of the total cytosolic proteins resolved by 2DGE had differing abundances in response to a switch from planktonic growth to surface-associated growth when the cells were cultivated in 1 mM Ca2+. At higher [Ca2+] this number increased to 38%. Fifteen cellular proteins that were differentially expressed in response to biofilm growth and/or Ca2+ were analysed by N-terminal sequencing and/or MS/MS. These proteins were identified as factors involved in cellular metabolic functions, putative proteases and transport proteins, although there were several proteins that had not been previously characterized. These results indicate that Ca2+ causes global changes in matrix material, as well as in cellular and extracellular protein profiles of Pseudoalteromonas sp. 1398. These changes are more pronounced when the bacterium grows in biofilms than when it grows in planktonic culture.en_US
dc.titleCalcium influences cellular and extracellular product formation during biofilm-associated growth of a marine Pseudoalteromonas sp.en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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