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dc.contributor.authorKjellerup, B. V.
dc.contributor.authorVeeh, Richard Harold
dc.contributor.authorSumithrarathne, P.
dc.contributor.authorThomsen, T. R.
dc.contributor.authorBuckingham-Meyer, Kelli
dc.contributor.authorFrolund, B.
dc.contributor.authorSturman, Paul J.
dc.date.accessioned2017-07-19T22:42:22Z
dc.date.available2017-07-19T22:42:22Z
dc.date.issued2005-04
dc.identifier.citationKjellerup BV, Veeh RH, Sumithraratne P, Thomsen TR, Buckingham-Meyer K, Frolund B, Sturman P, "Monitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniques," J Ind Microbiol Biotechnol, 2005 32:163-170en_US
dc.identifier.issn1367-5435
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13348
dc.description.abstractThe identification of bacteria in oil production facilities has previously been based on culture techniques. However, cultivation of bacteria from these often-extreme environments can lead to errors in identifying the microbial community members. In this study, molecular techniques including fluorescence in situ hybridization, PCR, denaturing gradient gel electrophoresis, and sequencing were used to track changes in bacterial biofilm populations treated with nitrate, nitrite, or nitrate+molybdate as agents for the control of sulfide production. Results indicated that nitrite and nitrate+molybdate reduced sulfide production, while nitrate alone had no effect on sulfide generation. No long-term effect on sulfide production was observed. Initial sulfate-reducing bacterial numbers were not influenced by the chemical treatments, although a significant increase in sulfate-reducing bacteria was observed after termination of the treatments. Molecular analysis showed a diverse bacterial population, but no major shifts in the population due to treatment effects were observed.en_US
dc.titleMonitoring of microbial souring in chemically treated, produced-water biofilm systems using molecular techniquesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage163en_US
mus.citation.extentlastpage170en_US
mus.citation.issue4en_US
mus.citation.journaltitleJournal of Industrial Microbiology & Biotechnologyen_US
mus.citation.volume32en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s10295-005-0222-5en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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