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dc.contributor.authorMarion-Ferey, Karine
dc.contributor.authorLeid, Jeff G.
dc.contributor.authorBouvier, Ghislaine
dc.contributor.authorPasmore, M.
dc.contributor.authorHusson, Gilles
dc.contributor.authorVillagines, Rolland
dc.identifier.citationMarion-Ferey K, Leid J, Bouvier G, Pasmore M, Husson G, Vilagines R, "Endotoxin level measurement in hemodialysis biofilm using the whole blood assay," Artif Organs, 2005 29(6):475-481en_US
dc.description.abstractBiofilms have been found on the inner surface of silicone tubing inside dialysis machines. Endotoxin releasing from those biofilms increases the bioincompatibility of dialysis liquids and leads to long-term inflammatory complications among dialysis patients. Endotoxin measurement is recommended for the control of dialysis liquids. This article describes the use of a new method, the Whole Blood Assay (WBA), for endotoxin quantification in dialysis biofilms. Biofilms were suspended in sterile water by scraping the tubing samples. Diluted blood samples from healthy donors were stimulated overnight with the contaminated suspension. Stimulated mononuclear cells released IL-1β in response to endotoxins. IL-1β level was then measured using an ultrasensitive ELISA method. We demonstrated a semilogarithmic model in which the optical densities measured after the ELISA assay increases linearly with the levels of endotoxin. This model allowed the determination of the amount of endotoxins in biofilm samples with a detection limit of 0.032 EU/mL. Most of the time, the amounts of endotoxin measured by the WBA were higher than those measured by the Limulus Amoebocyte Lysate (LAL) assay. This study suggested the presence of “endotoxin-like” compounds different from the lipopolysaccharides that are not detected by the LAL assay. We concluded that the LAL is necessary but insufficient to have a representative quantification of endotoxins that could be hazardous to patient health.en_US
dc.titleEndotoxin level measurement in hemodialysis biofilm using the whole blood assayen_US
mus.citation.journaltitleArtificial Organsen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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