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dc.contributor.authorAbdul Rani, Suriani
dc.contributor.authorPitts, Betsey
dc.contributor.authorStewart, Philip S.
dc.identifier.citationAbdul Rani S, Pitts B, Stewart PS, "Rapid diffusion of fluorescent tracers into Staphylococcus epidermidis biofilms visualized by time lapse microscopy," Antimicrob Agents Chemother, 2005 49(2):728-732en_US
dc.description.abstractThe transient diffusion of fluorescent tracers into biofilm cell clusters of Staphylococcus epidermidis was visualized by time lapse confocal scanning laser microscopy. Rhodamine B diffused into the center of cell clusters that were 200 to 600 micron in diameter within a few minutes. The apparent effective diffusion coefficient calculated from these data averaged 3.7 x 10(-7) cm(2) s(-1) or 11% of the value in pure water. Fluorescein diffused into biofilm more rapidly, with a diffusion coefficient that averaged 1.6 x 10(-6) cm(2) s(-1), or 32% of the value in water. This study provides direct, visual confirmation that solutes the size of many antibiotics and biocides can diffuse rapidly into biofilms.en_US
dc.titleRapid diffusion of fluorescent tracers into Staphylococcus epidermidis biofilms visualized by time lapse microscopyen_US
mus.citation.journaltitleAntimicrobial Agents and Chemotherapyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US

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