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dc.contributor.authorWerner, Erin M.
dc.contributor.authorRoe, Frank L.
dc.contributor.authorBugnicourt, Amandine
dc.contributor.authorFranklin, Michael J.
dc.contributor.authorHeydorn, Arne
dc.contributor.authorMolin, Søren
dc.contributor.authorPitts, Betsey
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-07-20T16:52:08Z
dc.date.available2017-07-20T16:52:08Z
dc.date.issued2004-10
dc.identifier.citationWerner E, Roe F, Bugnicourt A, Franklin MJ, Heydorn A, Molin S, Pitts B, Stewart PS, "Stratified growth in Pseudomonas aeruginosa biofilms," Appl Environ Microbiol, 2004 70(10):6188-6196en_US
dc.identifier.issn0099-2240
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13368
dc.description.abstractIn this study, stratified patterns of protein synthesis and growth were demonstrated in Pseudomonas aeruginosa biofilms. Spatial patterns of protein synthetic activity inside biofilms were characterized by the use of two green fluorescent protein (GFP) reporter gene constructs. One construct carried an isopropyl-ß-D-thiogalactopyranoside (IPTG)-inducible gfpmut2 gene encoding a stable GFP. The second construct carried a GFP derivative, gfp-AGA, encoding an unstable GFP under the control of the growth-rate-dependent rrnBp1 promoter. Both GFP reporters indicated that active protein synthesis was restricted to a narrow band in the part of the biofilm adjacent to the source of oxygen. The zone of active GFP expression was approximately 60 µm wide in colony biofilms and 30 µm wide in flow cell biofilms. The region of the biofilm in which cells were capable of elongation was mapped by treating colony biofilms with carbenicillin, which blocks cell division, and then measuring individual cell lengths by transmission electron microscopy. Cell elongation was localized at the air interface of the biofilm. The heterogeneous anabolic patterns measured inside these biofilms were likely a result of oxygen limitation in the biofilm. Oxygen microelectrode measurements showed that oxygen only penetrated approximately 50 µm into the biofilm. P. aeruginosa was incapable of anaerobic growth in the medium used for this investigation. These results show that while mature P. aeruginosa biofilms contain active, growing cells, they can also harbor large numbers of cells that are inactive and not growing.en_US
dc.titleStratified growth in Pseudomonas aeruginosa biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage6188en_US
mus.citation.extentlastpage6196en_US
mus.citation.issue10en_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.citation.volume70en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aem.70.10.6188-6196.2004en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US


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