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dc.contributor.authorBeyenal, Haluk
dc.contributor.authorYakymyshyn, Chris
dc.contributor.authorHyungnak, Jeon
dc.contributor.authorDavis, Catherine C.
dc.contributor.authorLewandowski, Zbigniew
dc.date.accessioned2017-07-20T17:46:33Z
dc.date.available2017-07-20T17:46:33Z
dc.date.issued2004-09
dc.identifier.citationBeyenal H, Yakymyshyn C, Hyungnak J, Davis CC, Lewandowski Z, "An optical microsensor to measure fluorescent light intensity in biofilms," J Microbiol Methods, 2004 58(3):367-374en_US
dc.identifier.issn0167-7012
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13373
dc.description.abstractWe have developed an optical microsensor to quantify fluorescent light intensity distribution in biofilms. The optical system consisted of a beam splitter, light couplers, filters and a spectrophotometer able to accept the fiberoptic cable to measure fluorescent light intensity. The emitted light, fluorescence from the biofilm, was collected at the tip of the optical microsensor and was transferred to a spectrophotometer via a fiberoptic cable. The total fluorescent light intensity was evaluated from the emission spectrum by numerical integration. The newly developed fiberoptic microsensor was tested using a Staphylococcus aureus strain producing yellow fluorescent protein (YFP) grown as biofilm. We used a 405-nm violet laser diode for excitation, and measured the emission intensity between 480 nm and 540 nm. The optical microsensor that quantifies fluorescent light intensity is a promising tool in biofilm research which often requires detection and quantification of fluorescent light intensity distribution generated by various fluorescent proteins.en_US
dc.titleAn optical microsensor to measure fluorescent light intensity in biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage367en_US
mus.citation.extentlastpage374en_US
mus.citation.issue3en_US
mus.citation.journaltitleJournal of Microbiological Methodsen_US
mus.citation.volume58en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1016/j.mimet.2004.05.003en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US


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