Show simple item record

dc.contributor.authorPurevdorj, B.
dc.contributor.authorStoodley, Paul
dc.date.accessioned2017-07-20T20:03:10Z
dc.date.available2017-07-20T20:03:10Z
dc.date.issued2004
dc.identifier.citationPurevdorj B, Stoodley P, "Biofilm structure, behavior, and hydrodynamics," In: Microbial Biofilms, Ghannoum MA, O'Toole (eds), 2004 ASM Press, Washington, DC, pp 160-173en_US
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13383
dc.description.abstractHexavalent uranium [U(VI)] was immobilized using biofilms of the sulfate-reducing bacterium (SRB) Desulfovibrio desulfuricans G20. The biofilms were grown in flat-plate continuous-flow reactors using lactate as the electron donor and sulfate as the electron acceptor. U(VI)was continuously fed into the reactor for 32 weeks at a concentration of 126 microM. During this time, the soluble U(VI) was removed (between 88 and 96% of feed) from solution and immobilized in the biofilms. The dynamics of U immobilization in the sulfate-reducing biofilms were quantified by estimating: (1) microbial activity in the SRB biofilm, defined as the hydrogen sulfide (H2S) production rate and estimated from the H2S concentration profiles measured using microelectrodes across the biofilms; (2) concentration of dissolved U in the solution; and (3) the mass of U precipitated in the biofilm. Results suggest that U was immobilized in the biofilms as a result of two processes: (1) enzymatically and (2) chemically, by reacting with microbially generated H2S. Visual inspection showed that the dissolved sulfide species reacted with U(VI) to produce a black precipitate. Synchrotron-based U L3-edge X-ray absorption near edge structure (XANES) spectroscopy analysis of U precipitated abiotically by sodium sulfide indicated that U(VI) had been reduced to U(IV). Selected-area electron diffraction pattern and crystallographic analysis of transmission electron microscope lattice-fringe images confirmed the structure of precipitated U as being that of uraninite.en_US
dc.titleBiofilm structure, behavior, and hydrodynamicsen_US
dc.typeBook chapteren_US
mus.citation.extentfirstpage160en_US
mus.citation.extentlastpage173en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/9781555817718.ch9en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US
mus.citation.booktitleMicrobial Biofilmsen_US
mus.contributor.orcidStoodley, Paul|0000-0001-6069-273Xen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.