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dc.contributor.authorBeyenal, Haluk
dc.contributor.authorSani, Rajesh K.
dc.contributor.authorPeyton, Brent M.
dc.contributor.authorDohnalkova, Alice
dc.contributor.authorAmonette, James E.
dc.contributor.authorLewandowski, Zbigniew
dc.date.accessioned2017-07-20T20:16:37Z
dc.date.available2017-07-20T20:16:37Z
dc.date.issued2004-04
dc.identifier.citationBeyenal H, Sani RK, Peyton BM, Dohnalkova AC, Amonette JE, Lewandowski Z, "Uranium immobilization by sulfate-reducing biofilms," Environ Sci Technol, 2004 38(7):2067-2074en_US
dc.identifier.issn0013-936X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13386
dc.description.abstractHexavalent uranium [U(VI)] was immobilized using biofilms of the sulfate-reducing bacterium (SRB) Desulfovibrio desulfuricans G20. The biofilms were grown in flat-plate continuous-flow reactors using lactate as the electron donor and sulfate as the electron acceptor. U(VI)was continuously fed into the reactor for 32 weeks at a concentration of 126 microM. During this time, the soluble U(VI) was removed (between 88 and 96% of feed) from solution and immobilized in the biofilms. The dynamics of U immobilization in the sulfate-reducing biofilms were quantified by estimating: (1) microbial activity in the SRB biofilm, defined as the hydrogen sulfide (H2S) production rate and estimated from the H2S concentration profiles measured using microelectrodes across the biofilms; (2) concentration of dissolved U in the solution; and (3) the mass of U precipitated in the biofilm. Results suggest that U was immobilized in the biofilms as a result of two processes: (1) enzymatically and (2) chemically, by reacting with microbially generated H2S. Visual inspection showed that the dissolved sulfide species reacted with U(VI) to produce a black precipitate. Synchrotron-based U L3-edge X-ray absorption near edge structure (XANES) spectroscopy analysis of U precipitated abiotically by sodium sulfide indicated that U(VI) had been reduced to U(IV). Selected-area electron diffraction pattern and crystallographic analysis of transmission electron microscope lattice-fringe images confirmed the structure of precipitated U as being that of uraninite.en_US
dc.titleUranium immobilization by sulfate-reducing biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage2067en_US
mus.citation.extentlastpage2074en_US
mus.citation.issue7en_US
mus.citation.journaltitleEnvironmental Science & Technologyen_US
mus.citation.volume38en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1021/es0348703en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCell Biology & Neuroscience.en_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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