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dc.contributor.authorRoberts, M. E.
dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-07-20T21:21:50Z
dc.date.available2017-07-20T21:21:50Z
dc.date.issued2003-12
dc.identifier.citationRoberts ME, Stewart PS, "Modeling antibiotic tolerance in biofilms by accounting for nutrient limitation," Antimicrob Agents Chemother, 2004 48(1):48-52en_US
dc.identifier.issn0066-4804
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13395
dc.description.abstractA mathematical model of biofilm dynamics was used to investigate the protection from antibiotic killing that can be afforded to microorganisms in biofilms based on a mechanism of localized nutrient limitation and slow growth. The model assumed that the rate of killing by the antibiotic was directly proportional to the local growth rate. Growth rates in the biofilm were calculated by using the local concentration of a single growth-limiting substrate with Monod kinetics. The concentration profile of this metabolic substrate was calculated by solving a reaction-diffusion problem. The model predicted the following features: stratified patterns of growth with zones of no growth in the biofilm interior, slow killing of biofilm microorganisms that was further retarded as the initial biofilm thickness increased, nonuniform spatial patterns of killing inside the biofilm, biofilm killing rates that decrease in a nonlinear way as the concentration of the growth-limiting substrate feeding the biofilm is decreased, and heightened tolerance when external mass transfer resistance is manifested. This modeling study also provides motivation for further investigation of a hypothetical cell state in which damaged cells score as nonviable but continue to consume substrate. The existence of such a cell state can further retard biofilm killing, according to the simulations. The results support the important contributions of nutrient limitation and slow growth to the antibiotic tolerance of microorganisms in biofilms.en_US
dc.titleModeling antibiotic tolerance in biofilms by accounting for nutrient limitationen_US
dc.typeArticleen_US
mus.citation.extentfirstpage48en_US
mus.citation.extentlastpage52en_US
mus.citation.issue1en_US
mus.citation.journaltitleAntimicrobial Agents and Chemotherapyen_US
mus.citation.volume48en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aac.48.1.48-52.2004en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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