Montana State University (MSU) Library in Bozeman Montana State University - Home Montana State University Libraries - Home
    • Login
    View Item 
    •   ScholarWorks Home
    • Scholarship & Research
    • Publications by Colleges and Departments (MSU- Bozeman)
    • College of Engineering
    • Center for Biofilm Engineering (CBE)
    • Scholarly Work - Center for Biofilm Engineering
    • View Item
    •   ScholarWorks Home
    • Scholarship & Research
    • Publications by Colleges and Departments (MSU- Bozeman)
    • College of Engineering
    • Center for Biofilm Engineering (CBE)
    • Scholarly Work - Center for Biofilm Engineering
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Statistical quantification of the detachment rates and size distribution of cell clumps from wild type (PAO1) and cell signaling mutant (JP1) Pseudomonas aeruginosa biofilms

    Thumbnail
    View/Open
    Published article (345.5Kb)
    Date
    2004-10
    Author
    Wilson, Suzanne
    Hamilton, Martin A.
    Hamilton, G. C.
    Schumann, M. R.
    Stoodley, Paul
    Metadata
    Show full item record
    Abstract
    The detachment of cells from bacterial biofilms is an important, yet poorly understood and largely unquantified phenomenon. Detached cell clumps from medical devices may form microemboli and lead to metastasis, especially if they are resistant to host defenses and antibiotics. In manufacturing plants detached clumps entering a process stream decrease product quality. Two strains of Pseudomonas aeruginosa, a wild type (PAO1) and a cell signaling mutant (JP1), were studied to (i) quantify and model detachment patterns and (ii) determine the influence of cell signaling on detachment. We collected effluent from a biofilm flowthrough reactor and determined the size distribution for cell detachment events by microscopic examination and image analysis. The two strains were similar in terms of both biofilm structure and detachment patterns. Most of the detachment events were single-cell events; however, multiple-cell detachment events contributed a large fraction of the total detached cells. The rates at which events containing multiple cells detached from the biofilm were estimated by fitting a statistical model to the size distribution data. For events consisting of at least 1,000 cells, the estimated rates were 4.5 events mm–2 min–1 for PAO1 and 4.3 events mm–2 min–1 for JP1. These rates may be significant when they are scaled up to the total area of a real biofilm-contaminated medical device surface and to the hours or days of patient exposure.
    URI
    https://scholarworks.montana.edu/xmlui/handle/1/13429
    DOI
    10.1128/aem.70.10.5847-5852.2004
    Citation
    Wilson S, Hamilton MA, Hamilton GC, Schumann MR, Stoodley P, "Statistical quantification of the detachment rates and size distribution of cell clumps from wild type (PAO1) and cell signaling mutant (JP1) Pseudomonas aeruginosa biofilms," Appl Environ Microbiol, 2004 70(10):5847-5852
    Collections
    • Scholarly Work - Center for Biofilm Engineering

    MSU uses DSpace software, copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback
     

     

    Browse

    All of ScholarWorksCommunities & CollectionsBy Issue DateAuthorsTitlesDepartmentsItem TypeThis CollectionBy Issue DateAuthorsTitlesDepartmentsItem Type

    My Account

    Login

    Guidelines & Policies

    AllFor authorsWhy to submitHow to submit

    Statistics

    View Usage Statistics

    MSU uses DSpace software, copyright © 2002-2013  Duraspace
    Contact Us | Send Feedback