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dc.contributor.authorWilson, Suzanne
dc.contributor.authorHamilton, Martin A.
dc.contributor.authorHamilton, G. C.
dc.contributor.authorSchumann, M. R.
dc.contributor.authorStoodley, Paul
dc.date.accessioned2017-07-27T15:38:43Z
dc.date.available2017-07-27T15:38:43Z
dc.date.issued2004-10
dc.identifier.citationWilson S, Hamilton MA, Hamilton GC, Schumann MR, Stoodley P, "Statistical quantification of the detachment rates and size distribution of cell clumps from wild type (PAO1) and cell signaling mutant (JP1) Pseudomonas aeruginosa biofilms," Appl Environ Microbiol, 2004 70(10):5847-5852en_US
dc.identifier.issn0099-2240
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13429
dc.description.abstractThe detachment of cells from bacterial biofilms is an important, yet poorly understood and largely unquantified phenomenon. Detached cell clumps from medical devices may form microemboli and lead to metastasis, especially if they are resistant to host defenses and antibiotics. In manufacturing plants detached clumps entering a process stream decrease product quality. Two strains of Pseudomonas aeruginosa, a wild type (PAO1) and a cell signaling mutant (JP1), were studied to (i) quantify and model detachment patterns and (ii) determine the influence of cell signaling on detachment. We collected effluent from a biofilm flowthrough reactor and determined the size distribution for cell detachment events by microscopic examination and image analysis. The two strains were similar in terms of both biofilm structure and detachment patterns. Most of the detachment events were single-cell events; however, multiple-cell detachment events contributed a large fraction of the total detached cells. The rates at which events containing multiple cells detached from the biofilm were estimated by fitting a statistical model to the size distribution data. For events consisting of at least 1,000 cells, the estimated rates were 4.5 events mm–2 min–1 for PAO1 and 4.3 events mm–2 min–1 for JP1. These rates may be significant when they are scaled up to the total area of a real biofilm-contaminated medical device surface and to the hours or days of patient exposure.en_US
dc.titleStatistical quantification of the detachment rates and size distribution of cell clumps from wild type (PAO1) and cell signaling mutant (JP1) Pseudomonas aeruginosa biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage5847en_US
mus.citation.extentlastpage5852en_US
mus.citation.issue10en_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.citation.volume70en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aem.70.10.5847-5852.2004en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US
mus.contributor.orcidStoodley, Paul|0000-0001-6069-273Xen_US


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