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dc.contributor.authorMah, Thien-Fah
dc.contributor.authorPitts, Betsey
dc.contributor.authorPellock, Brett
dc.contributor.authorWalker, Graham C.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorO'Toole, George A.
dc.date.accessioned2017-08-08T22:01:40Z
dc.date.available2017-08-08T22:01:40Z
dc.date.issued2003-11
dc.identifier.citationMahT-F, Pitts B, Pellock B, Walker GC, Stewart PS and O’Toole GA, "A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance," Nature, 2003 426(6964):306–310.en_US
dc.identifier.issn0028-0836
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13469
dc.description.abstractBiofilms are surface-attached microbial communities with characteristic architecture and phenotypic and biochemical properties distinct from their free-swimming, planktonic counterparts. One of the best-known of these biofilm-specific properties is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. We report a genetic determinant of this high-level resistance in the Gram-negative opportunistic pathogen, Pseudomonas aeruginosa. We have identified a mutant of P. aeruginosa that, while still capable of forming biofilms with the characteristic P. aeruginosa architecture, does not develop high-level biofilm-specific resistance to three different classes of antibiotics. The locus identified in our screen, ndvB, is required for the synthesis of periplasmic glucans. Our discovery that these periplasmic glucans interact physically with tobramycin suggests that these glucose polymers may prevent antibiotics from reaching their sites of action by sequestering these antimicrobial agents in the periplasm. Our results indicate that biofilms themselves are not simply a diffusion barrier to these antibiotics, but rather that bacteria within these microbial communities employ distinct mechanisms to resist the action of antimicrobial agents.en_US
dc.titleA genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistanceen_US
dc.typeArticleen_US
mus.citation.extentfirstpage306en_US
mus.citation.extentlastpage310en_US
mus.citation.issue6964en_US
mus.citation.journaltitleNatureen_US
mus.citation.volume426en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1038/nature02122en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage2en_US


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