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    Compromised host defense on Pseudomonas aeruginosa biofilms: Characterization of neutrophil and biofilm interactions

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    Published article (1.030Mb)
    Date
    2003-10
    Author
    Jesaitis, A. J.
    Franklin, Michael J.
    Berglund, Deborah L.
    Sasaki, Maiko
    Lord, Connie I.
    Bleazard, Justin Brock
    Duffy, James E.
    Beyenal, Haluk
    Lewandowski, Zbigniew
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    Abstract
    Pseudomonas aeruginosa is an opportunistic pathogen that forms biofilms on tissues and other surfaces. We characterized the interaction of purified human neutrophils with P. aeruginosa, growing in biofilms, with regard to morphology, oxygen consumption, phagocytosis, and degranulation. Scanning electron and confocal laser microscopy indicated that the neutrophils retained a round, unpolarized, unstimulated morphology when exposed to P. aeruginosa PAO1 biofilms. However, transmission electron microscopy demonstrated that neutrophils, although rounded on their dorsal side, were phagocytically active with moderate membrane rearrangement on their bacteria-adjacent surfaces. The settled neutrophils lacked pseudopodia, were impaired in motility, and were enveloped by a cloud of planktonic bacteria released from the biofilms. The oxygen consumption of the biofilm/neutrophil system increased 6- and 8-fold over that of the biofilm alone or unstimulated neutrophils in suspension, respectively. H(2)O(2) accumulation was transient, reaching a maximal measured value of 1 micro M. Following contact, stimulated degranulation was 20-40% (myeloperoxidase, beta-glucuronidase) and 40-80% (lactoferrin) of maximal when compared with formylmethionylleucylphenylalanine plus cytochalasin B stimulation. In summary, after neutrophils settle on P. aeruginosa biofilms, they become phagocytically engorged, partially degranulated, immobilized, and rounded. The settling also causes an increase in oxygen consumption of the system, apparently resulting from a combination of a bacterial respiration and escape response and the neutrophil respiratory burst but with little increase in the soluble concentration of H(2)O(2). Thus, host defense becomes compromised as biofilm bacteria escape while neutrophils remain immobilized with a diminished oxidative potential.
    URI
    https://scholarworks.montana.edu/xmlui/handle/1/13474
    DOI
    10.4049/jimmunol.171.8.4329
    Citation
    Jesaitis AJ, Franklin MJ, Berglund D, Sasaki M, Lord CI, Bleazard J, Duffy JE, Beyenal H, Lewandowski Z, "Compromised host defense on Pseudomonas aeruginosa biofilms: Characterization of neutrophil and biofilm interactions," J Immunol, 2003 171(8):4329-4339.
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