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dc.contributor.authorJesaitis, A. J.
dc.contributor.authorFranklin, Michael J.
dc.contributor.authorBerglund, Deborah L.
dc.contributor.authorSasaki, Maiko
dc.contributor.authorLord, Connie I.
dc.contributor.authorBleazard, Justin Brock
dc.contributor.authorDuffy, James E.
dc.contributor.authorBeyenal, Haluk
dc.contributor.authorLewandowski, Zbigniew
dc.identifier.citationJesaitis AJ, Franklin MJ, Berglund D, Sasaki M, Lord CI, Bleazard J, Duffy JE, Beyenal H, Lewandowski Z, "Compromised host defense on Pseudomonas aeruginosa biofilms: Characterization of neutrophil and biofilm interactions," J Immunol, 2003 171(8):4329-4339.en_US
dc.description.abstractPseudomonas aeruginosa is an opportunistic pathogen that forms biofilms on tissues and other surfaces. We characterized the interaction of purified human neutrophils with P. aeruginosa, growing in biofilms, with regard to morphology, oxygen consumption, phagocytosis, and degranulation. Scanning electron and confocal laser microscopy indicated that the neutrophils retained a round, unpolarized, unstimulated morphology when exposed to P. aeruginosa PAO1 biofilms. However, transmission electron microscopy demonstrated that neutrophils, although rounded on their dorsal side, were phagocytically active with moderate membrane rearrangement on their bacteria-adjacent surfaces. The settled neutrophils lacked pseudopodia, were impaired in motility, and were enveloped by a cloud of planktonic bacteria released from the biofilms. The oxygen consumption of the biofilm/neutrophil system increased 6- and 8-fold over that of the biofilm alone or unstimulated neutrophils in suspension, respectively. H(2)O(2) accumulation was transient, reaching a maximal measured value of 1 micro M. Following contact, stimulated degranulation was 20-40% (myeloperoxidase, beta-glucuronidase) and 40-80% (lactoferrin) of maximal when compared with formylmethionylleucylphenylalanine plus cytochalasin B stimulation. In summary, after neutrophils settle on P. aeruginosa biofilms, they become phagocytically engorged, partially degranulated, immobilized, and rounded. The settling also causes an increase in oxygen consumption of the system, apparently resulting from a combination of a bacterial respiration and escape response and the neutrophil respiratory burst but with little increase in the soluble concentration of H(2)O(2). Thus, host defense becomes compromised as biofilm bacteria escape while neutrophils remain immobilized with a diminished oxidative potential.en_US
dc.titleCompromised host defense on Pseudomonas aeruginosa biofilms: Characterization of neutrophil and biofilm interactionsen_US
mus.citation.journaltitleJournal of Immunologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.departmentMicrobiology & Immunology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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