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dc.contributor.authorMarion-Ferey, Karine
dc.contributor.authorEnkiri, F.
dc.contributor.authorPasmore, M.
dc.contributor.authorHusson, Gilles
dc.contributor.authorVilagines, Rolland
dc.date.accessioned2017-08-09T22:25:34Z
dc.date.available2017-08-09T22:25:34Z
dc.date.issued2003-07
dc.identifier.citationMarion-Ferey, K., F. Enkiri, M. Pasmore, G.P. Husson, and R. Vilagines, "Methods for biofilm analysis on silicone tubing of dialysis machines," Artif. Organs, 27(7):658-664 (2003)en_US
dc.identifier.issn0160-564X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13492
dc.description.abstractWe describe an analytical protocol to study biofilms that develop inside silicone tubing of dialysis machines. This protocol has been set up with the help of a dynamic testing device reproducing dialysis conditions. The methodology includes direct microscopic observation, biofilm removal with an original mechanical biofilm scraper, quantitative analysis with culturable and total bacteria counting, and endotoxin level measurement using the LAL chromogenic kinetic assay. The analytical protocol has been assessed on 13 different clinical tubing samples. Most samples were contaminated by adherent cells and the thickest biofilms were found at the connection between the dialysis water distribution loop and the dialysis machine. The less contaminated samples had been removed from dialysis machines that were decontaminated with citric acid and autoclaving, showing the importance of the decontamination procedure for the prevention of biofilm development. This article shows that easy, rapid, reproducible, and economical methods are applicable for a routine analysis of biofilms that develop on dialysis systems and should be included in the regular control of the microbiological quality of dialysis liquids.en_US
dc.titleMethods for biofilm analysis on silicone tubing of dialysis machinesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage658en_US
mus.citation.extentlastpage664en_US
mus.citation.issue7en_US
mus.citation.journaltitleArtificial Organsen_US
mus.citation.volume27en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1046/j.1525-1594.2003.07148.xen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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