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dc.contributor.authorIshaq, Suzanne L.
dc.contributor.authorJohnson, Stephen P.
dc.contributor.authorMiller, Zach J.
dc.contributor.authorLehnhoff, Erik A.
dc.contributor.authorOlivo, Sarah K.
dc.contributor.authorYeoman, Carl J.
dc.contributor.authorMenalled, Fabian D.
dc.date.accessioned2017-08-15T21:45:05Z
dc.date.available2017-08-15T21:45:05Z
dc.date.issued2017-02
dc.identifier.citationIshaq, Suzanne L. , Stephen P. Johnson, Zach J. Miller, Erik A. Lehnhoff, Sarah Olivo, Carl J. Yeoman, and Fabian D. Menalled. "Impact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structure." Microbial Ecology 73, no. 2 (February 2017): 417-434. DOI: 10.1007/s00248-016-0861-2.en_US
dc.identifier.issn1432-184X
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13514
dc.description.abstractFarming practices affect the soil microbial community, which in turn impacts crop growth and crop-weed interactions. This study assessed the modification of soil bacterial community structure by organic or conventional cropping systems, weed species identity [Amaranthus retroflexus L. (redroot pigweed) or Avena fatua L. (wild oat)], and living or sterilized inoculum. Soil from eight paired USDA-certified organic and conventional farms in north-central Montana was used as living or autoclave-sterilized inoculant into steam-pasteurized potting soil, planted with Am. retroflexus or Av. fatua and grown for two consecutive 8-week periods to condition soil nutrients and biota. Subsequently, the V3-V4 regions of the microbial 16S rRNA gene were sequenced by Illumina MiSeq. Treatments clustered significantly, with living or sterilized inoculum being the strongest delineating factor, followed by organic or conventional cropping system, then individual farm. Living inoculum-treated soil had greater species richness and was more diverse than sterile inoculum-treated soil (observed OTUs, Chao, inverse Simpson, Shannon, P<0.001) and had more discriminant taxa delineating groups (linear discriminant analysis). Living inoculum soil contained more Chloroflexi and Acidobacteria, while the sterile inoculum soil had more Bacteroidetes, Firmicutes, Gemmatimonadetes, and Verrucomicrobia. Organically farmed inoculum-treated soil had greater species richness, more diversity (observed OTUs, Chao, Shannon, P<0.05), and more discriminant taxa than conventionally farmed inoculum-treated soil. Cyanobacteria were higher in pots growing Am. retroflexus, regardless of inoculum type, for three of the four organic farms. Results highlight the potential of cropping systems and species identity to modify soil bacterial communities, subsequently modifying plant growth and crop-weed competition.en_US
dc.description.sponsorshipUSDA-OREI (Grant MONB00365); USDA-ORG (2011-04960); Montana University System Research Initiative: 51040-MUSRI2015-02en_US
dc.titleImpact of Cropping Systems, Soil Inoculum, and Plant Species Identity on Soil Bacterial Community Structureen_US
dc.typeArticleen_US
mus.citation.extentfirstpage417en_US
mus.citation.extentlastpage434en_US
mus.citation.issue2en_US
mus.citation.journaltitleMicrobial Ecologyen_US
mus.citation.volume73en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1007/s00248-016-0861-2en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage6en_US


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