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dc.contributor.authorDoublet, Vincent
dc.contributor.authorPoeschl, Yvonne
dc.contributor.authorGogol-Doering, Andreas
dc.contributor.authorAlaux, Cedric
dc.contributor.authorAnnoscia, Desiderato
dc.contributor.authorAurori, Christian
dc.contributor.authorBarribeau, Seth M.
dc.contributor.authorBedoya-Reina, Oscar C.
dc.contributor.authorBrown, Mark J. F.
dc.contributor.authorBull, James C.
dc.contributor.authorFlenniken, Michelle L.
dc.contributor.authorGalbraith, David A.
dc.contributor.authorGenersch, Elke
dc.contributor.authorGisder, Sebastian
dc.contributor.authorGrosse, Ivo
dc.contributor.authorHolt, Holly
dc.contributor.authorHultmark, Dan
dc.contributor.authorLattorff, H. Michael G.
dc.contributor.authorLe Conte, Yves
dc.contributor.authorManfredini, Fabio
dc.contributor.authorMcMahon, Dino P.
dc.identifier.citationDoublet, Vincent, Yvonne Poeschl, Andreas Gogol-Doering, Cedric Alaux, Desiderato Annoscia, Christian Aurori, Seth M. Barribeau, Oscar C. Bedoya-Reina, Mark J. F. Brown, James C. Bull, Michelle L. Flenniken, David A. Galbraith, Elke Genersch, Sebastian Gisder, Ivo Grosse, Holly L. Holt, Dan Hultmark, H. Michael G. Lattorff, Yves Le Conte, Fabio Manfredini, Dino P. McMahon, Robin F. A. Moritz, Francesco Nazzi, Elina L. Nino, Katja Nowick, Ronald P. van Rij, Robert J. Paxton, and Christina M. Grozinger. "Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens." BMC Genomics 18, no. 256 (March 2017).
dc.description.abstractBackground Organisms typically face infection by diverse pathogens, and hosts are thought to have developed specific responses to each type of pathogen they encounter. The advent of transcriptomics now makes it possible to test this hypothesis and compare host gene expression responses to multiple pathogens at a genome-wide scale. Here, we performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets. Thereby, we identified common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites: the Microsporidia Nosema apis and Nosema ceranae, RNA viruses, and the ectoparasitic mite Varroa destructor, which transmits viruses. Results We identified a common suite of genes and conserved molecular pathways that respond to all investigated pathogens, a result that suggests a commonality in response mechanisms to diverse pathogens. We found that genes differentially expressed after infection exhibit a higher evolutionary rate than non-differentially expressed genes. Using our new bioinformatics approach, we unveiled additional pathogen-specific responses of honey bees; we found that apoptosis appeared to be an important response following microsporidian infection, while genes from the immune signalling pathways, Toll and Imd, were differentially expressed after Varroa/virus infection. Finally, we applied our bioinformatics approach and generated a gene co-expression network to identify highly connected (hub) genes that may represent important mediators and regulators of anti-pathogen responses. Conclusions Our meta-analysis generated a comprehensive overview of the host metabolic and other biological processes that mediate interactions between insects and their pathogens. We identified key host genes and pathways that respond to phylogenetically diverse pathogens, representing an important source for future functional studies as well as offering new routes to identify or generate pathogen resilient honey bee stocks. The statistical and bioinformatics approaches that were developed for this study are broadly applicable to synthesize information across transcriptomic datasets. These approaches will likely have utility in addressing a variety of biological questions.en_US
dc.description.sponsorshipGerman Centre for Integrative Biodiversity Research; German Science Foundation (FZT 118); Biotechnology and Biological Sciences Research Council; Department for Environment, Food and Rural Affairs; Natural Environment Research Council; Scottish Governmenten_US
dc.titleUnity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogensen_US
mus.citation.journaltitleBMC Genomicsen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidFlenniken, Michelle L.|0000-0003-0356-3370en_US

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