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dc.contributor.authorEhrlich, Garth D.
dc.contributor.authorVeeh, Richard Harold
dc.contributor.authorWang, Xue
dc.contributor.authorCosterton, J. William
dc.contributor.authorHayes, Jay D.
dc.contributor.authorHu, Fen Z.
dc.contributor.authorDaigle, Bernie J.
dc.contributor.authorEhrlich, Miles D.
dc.contributor.authorPost, J. C.
dc.identifier.citationEhrlich GD, Veeh R, Wang X, Costerton JW, Hayes JD, Hu FZ, Daigle BJ, Ehrlich MD, Post JC, "Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis media," JAMA, 2002 287(13):1710-1715.en_US
dc.description.abstractCONTEXT Chronic otitis media with effusion (OME) has long been considered to be a sterile inflammatory process. The previous application of molecular diagnostic technologies to OME suggests that viable bacteria are present in complex communities known as mucosal biofilms; however, direct imaging evidence of mucosal biofilms associated with OM is lacking. OBJECTIVE. To determine whether biofilm formation occurs in middle-ear mucosa in an experimental model of otitis media. DESIGN and MATERIALS. A total of 48 research-grade, young adult chinchillas weighing 500g were used for 2 series of animal experiments: one to obtain specimens for scanning electron microscopy and the other to obtain specimens for confocal laser scanning microscopy using vital dyes. In each series, 21 animals were bilaterally injected with viable Haemophilus influenzae bacteria and 1 was inoculated to account for expected mortality. Three served as negative controls. Effusions and mucosal specimens were collected from 2 infected animals that were euthanized at 3, 6, 12, and 24 hours and at days 2, 4, 5, 10, 16, and 22 after inoculation. MAIN OUTCOME MEASURES. Images were analyzed for biofilm morphology, including presence of microcolony formation and for presence of bacteria on tissue surfaces. RESULTS. Scanning electron microscopy demonstrated that biofilm formation was evident in all specimens from animals beginning 1 day after infection and was present through 21 days. Confocal laser scanning microscopy indicated that bacteria within the biofilms was viable. CONCLUSION. These preliminary findings provide evidence that mucosal biofilms form in an experimental model of otitis media and suggest that biofilm formation may be an important factor in the pathogenesis of chronic otitis media with effusion.en_US
dc.titleMucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis mediaen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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