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dc.contributor.authorSauer, K.
dc.contributor.authorCamper, Anne K.
dc.contributor.authorEhrlich, Garth D.
dc.contributor.authorCosterton, J. William
dc.contributor.authorDavies, David Gwilym
dc.date.accessioned2017-08-21T20:50:24Z
dc.date.available2017-08-21T20:50:24Z
dc.date.issued2002-02
dc.identifier.citationSauer K, Camper AK, Ehrlich GD, Costerton JW, and Davies DG, "Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm," Journal of Bacteriology, 2002 184(4):1140-1154.en_US
dc.identifier.issn0021-9193
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13565
dc.description.abstractComplementary approaches were employed to characterize transitional episodes in Pseudomonas aeruginosa biofilm development using direct observation and whole-cell protein analysis. Microscopy and in situ reporter gene analysis were used to directly observe changes in biofilm physiology and to act as signposts to standardize protein collection for two-dimensional electrophoretic analysis and protein identification in chemostat and continuous-culture biofilm-grown populations. Using these approaches, we characterized five stages of biofilm development: (i) reversible attachment, (ii) irreversible attachment, (iii) maturation-1, (iv) maturation-2, and (v) dispersion. Biofilm cells were shown to change regulation of motility, alginate production, and quorum sensing during the process of development. The average difference in detectable protein regulation between each of the five stages of development was 35% (approximately 525 proteins). When planktonic cells were compared with maturation-2 stage biofilm cells, more than 800 proteins were shown to have a sixfold or greater change in expression level (over 50% of the proteome). This difference was higher than when planktonic P. aeruginosa were compared with planktonic cultures of Pseudomonas putida. Las quorum sensing was shown to play no role in early biofilm development but was important in later stages. Biofilm cells in the dispersion stage were more similar to planktonic bacteria than to maturation-2 stage bacteria. These results demonstrate that P. aeruginosa displays multiple phenotypes during biofilm development and that knowledge of stage-specific physiology may be important in detecting and controlling biofilm growth.en_US
dc.titlePseudomonas aeruginosa displays multiple phenotypes during development as a biofilmen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1140en_US
mus.citation.extentlastpage1154en_US
mus.citation.issue4en_US
mus.citation.journaltitleJournal of Bacteriologyen_US
mus.citation.volume184en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/jb.184.4.1140-1154.2002en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US


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