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dc.contributor.authorBaty, Ace M.
dc.contributor.authorEastburn, Callie C.
dc.contributor.authorDiwu, Zhenjun
dc.contributor.authorTechkarnjanaruk, Somkiet
dc.contributor.authorGoodman, Amanda E.
dc.contributor.authorGeesey, Gill G.
dc.date.accessioned2017-08-21T22:26:41Z
dc.date.available2017-08-21T22:26:41Z
dc.date.issued2000-08
dc.identifier.citationBaty, A.M., C.C. Eastburn, Z. Diwu, S. Techkarnjanaruk, A.E. Goodman, and G.G. Geesey, "Differentiation of Chitinase-Active and Non-Chitinase-Active Subpopulations of a Marine Bacterium during Dhitin Degradation," Appl. Environ. Microbiol., 66(8):353 (2000).en_US
dc.identifier.issn0099-2240
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13573
dc.description.abstractThe ability of marine bacteria to adhere to detrital particulate organic matter and rapidly switch on metabolic genes in an effort to reproduce is an important response for bacterial survival in the pelagic marine environment. The goal of this investigation was to evaluate the relationship between chitinolytic gene expression and extracellular chitinase activity in individual cells of the marine bacterium Pseudoalteromonas sp. strain S91 attached to solid chitin. A green fluorescent protein reporter gene under the control of the chiA promoter was used to evaluate chiA gene expression, and a precipitating enzyme-linked fluorescent probe, ELF-97-N-acetyl-b-D-glucosaminide, was used to evaluate extracellular chitinase activity among cells in the bacterial population. Evaluation of chiA expression and ELF-97 crystal location at the single-cell level revealed two physiologically distinct subpopulations of S91 on the chitin surface: one that was chitinase active and remained associated with the surface and another that was non-chitinase active and released daughter cells into the bulk aqueous phase. It is hypothesized that the surface-associated, non-chitinase-active population is utilizing chitin degradation products that were released by the adjacent chitinase-active populations for cell replication and dissemination into the bulk aqueous phase.en_US
dc.titleDifferentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradationen_US
dc.typeArticleen_US
mus.citation.extentfirstpage3566en_US
mus.citation.extentlastpage3573en_US
mus.citation.issue8en_US
mus.citation.journaltitleApplied and Environmental Microbiologyen_US
mus.citation.volume66en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aem.66.8.3566-3573.2000en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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