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dc.contributor.authorKern, Eric Anthony
dc.contributor.authorVeeh, Richard Harold
dc.contributor.authorMacur, Richard E.
dc.contributor.authorCunningham, Alfred B.
dc.date.accessioned2017-09-12T14:16:51Z
dc.date.available2017-09-12T14:16:51Z
dc.date.issued2002-01
dc.identifier.citationKern, E.A., R.H. Veeh, H.W. Langner, R.E. Macur, and A.B. Cunningham, "Characterization of Methyl tert-Butyl Ether-degrading Bacteria from a Gasoline-Contaminated Aquifer," Bioremediation J., 6(2):1 (2002).en_US
dc.identifier.issn1088-9868
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13613
dc.description.abstractMolecular microbial community analysis was combined with traditional cultivation strategies to investigate the presence of methyl tert-butyl ether (MTBE)-degrading bacteria in a gasoline-contaminated aquifer (Ronan, MT). A bacterial consortium, RS24, which is capable of complete mineralization of MTBE as a sole carbon and energy source was enriched from soil and aquifer materials taken from the contaminated site. The consortium was capable of degrading MTBE at rates up to 0.66 mg d-,1 with corresponding gross biomass yields of 0.25 ± 0.02 mg dry biomass (mg MTBE)-1. Two MTBE-degrading isolates identified as Pseudomonas Ant9 and Rhodococcus koreensis were obtained from the consortium. However, both isolates required the presence of 2-propanol as a cosubstrate for MTBE degradation. Denaturing gradient gel electrophoresis (DGGE) of Polymerase Chain Reaction (PCR)-amplified 16S rDNA confirmed the presence of both isolates in the initial consortium and indicated their disappearance with transfer and subculturing. MTBE degradation and cell growth by the consortium was stimulated by the presence of spent culture medium, suggesting the production of a growth factor during MTBE degradation. These results indicate the presence of naturally occurring MTBE-degrading bacteria in a contaminated aquifer and suggest the potential for natural attenuation or enhanced aerobic oxidation.en_US
dc.titleCharacterization of methyl tert-butyl ether-degrading bacteria from a gasoline-contaminated aquiferen_US
dc.typeArticleen_US
mus.citation.extentfirstpage113en_US
mus.citation.extentlastpage124en_US
mus.citation.issue2en_US
mus.citation.journaltitleBioremediation Journalen_US
mus.citation.volume6en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1080/10588330208951208en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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