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dc.contributor.authorNawaz, M. S.
dc.contributor.authorDavis, John W.
dc.contributor.authorWolfram, James H.
dc.contributor.authorChapatwala, Kirit D.
dc.date.accessioned2017-09-26T21:20:58Z
dc.date.available2017-09-26T21:20:58Z
dc.date.issued1991-03
dc.identifier.citationNawaz, M.S., J.W. Davis, J.H. Wolfram, and K.D. Chapatwala, "Degradation of organic cyanides by pseudomonas aeruginosa," Applied Biochemistry and Biotechnology, The Humana Press Inc., 28/29:865-875 (1991).en_US
dc.identifier.issn0273-2289
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/13768
dc.description.abstractA bacterium capable of utilizing acetonitrile (methyl cyanide) as the sole source of carbon and nitrogen was isolated from soil and identified asPseudomonas aeruginosa. This bacterium could also utilize and oxidize numerous lower-mol-wt nitrile compounds and their corresponding amides as growth substrates. A metabolite of acetonitrile in the culture medium was determined to be ammonia. The accumulation of ammonia in the culture medium was proportional to the concentration of the substrate and the inoculum. Cell extracts of the bacterium contained activities corresponding to nitrile aminohydrolase (E C 3.5.5.1) and amidase (E C 3.5.1.4), which regulate the degradation of acetonitrile. Both enzymes were inducible and hydrolyzed a wide range of substrates, and it was determined that the specific activity of amidase was far greater than the activity of nitrile aminohydrolase.en_US
dc.titleDegradation of organic cyanides by pseudomonas aeruginosaen_US
dc.typeArticleen_US
mus.citation.extentfirstpage865en_US
mus.citation.extentlastpage875en_US
mus.citation.issue1en_US
mus.citation.journaltitleApplied Biochemistry and Biotechnologyen_US
mus.citation.volume28-29en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/bf02922656en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage8en_US


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