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dc.contributor.authorPerea, K.
dc.contributor.authorPerz, Katharine A.
dc.contributor.authorOlivo, Sarah K.
dc.contributor.authorWilliams, Andrew F.
dc.contributor.authorLachman, Medora M.
dc.contributor.authorIshaq, Suzanne L.
dc.contributor.authorThomson, Jennifer M.
dc.contributor.authorYeoman, Carl J.
dc.identifier.citationPerea, K., K. Perz, S. K. Olivo, A. Williams, Medora Lachman, S. L. Ishaq, Jennifer Thomson, and Carl J. Yeoman. "Feed efficiency phenotypes in lambs involve changes in ruminal, colonic, and small-intestine-located microbiota." Journal of Animal Science 95, no. 6 (June 2017): 2585-2592. DOI: 10.2527/jas2016.1222.en_US
dc.description.abstractSeveral studies have revealed differences in rumen-located microbes between greatly efficient and inefficient animals; however, how the microbiota vary in the hind gastrointestinal tract (GIT) has only been sparsely explored and how they vary in the small intestine remains to be determined. We therefore sampled the microbiota of the duodenum, jejunum, ileum, colon, and colorectally-obtained feces, in addition to the rumen of 12 lambs that, in a residual feed intake trial, were found to be at either extreme of feed efficiency phenotypes. The 16S rRNA gene (V3-V4 region) profiles of all samples were analyzed and revealed unique microbiota in all GIT locations except the jejunum and ileum (ANOSIM R > 0.2, P < 0.001). Measures of beta-diversity revealed greater dissimilarity between more anatomically distant GIT locations (e.g., Rumen-Duodenum, ANOSIM R = 0.365, P < 0.001; Rumen-Colon, ANOSIM R = 1, P < 0.001) with the nearest distal region typically more similar than the nearest proximal location. The relative abundances of 13 operational taxonomic units (OTUs) from the duodenum, jejunum, colon, and feces, as well as the rumen, differed between efficient and inefficient animals (Bonferroni corrected, P < 0.05), while another 2 OTUs trended toward significance. These OTUs were classified as taxa with known roles in fibrolysis (Fibrobacteres, Ruminococcaceae, and Saccharofermentans) and others that are commonly associated with health (Bifidobacteriaceae, and Christensenellaceae) and dysbiosis (Proteobacteria). Our findings show biospatial delineations of microbiota throughout the GIT and suggest that feed efficiency extends beyond the rumen, transcending these regions, and involves increases in both rumen-and colon-located fibrolytic taxa, increases in bifidobacterial species in the small intestine, and reductions in small intestine and distal GIT-located Proteobacteria.en_US
dc.description.sponsorshipMontana Agricultural Experiment Station (project MONB00113); National Institute of General Medical Sciences of the National Institutes of Health (NIH-NIGMS: award number P20GM103474); Enhancing the Competitiveness of U.S. Beef (MONB00195); Multistate Research Project (W3177, W2010); Integrated Approach to Enhance Efficiency of Feed Utilization in Beef Production Systems (MONB00196)en_US
dc.titleFeed efficiency phenotypes in lambs involve changes in ruminal, colonic, and small-intestine-located microbiotaen_US
mus.citation.journaltitleJournal of Animal Scienceen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.contributor.orcidIshaq, Suzanne L.|0000-0002-2615-8055en_US
mus.contributor.orcidThomson, Jennifer M.|0000-0003-1921-0975en_US

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