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dc.contributor.authorBeech, Brian S.
dc.contributor.authorGaylarde, C. C.
dc.contributor.authorSmith, James J.
dc.contributor.authorGeesey, Gill G.
dc.identifier.citationBeech, B., C.C. Gaylarde, J.J. Smith, and G.G. Geesey, "Extracellular polysaccharides from desulfovibrio desulfuricans and pseudomonas fluorescens in the presence of mild and stainless steel," Appl. Microbiol. Biotechnol., 35(1):65-71 (1991).en_US
dc.description.abstractThis communication reports the presence of polysaccharides in biofilms formed by pure and mixed cultures of Desulfovibrio desulfuricans and Pseudomonas fluorescens on mild and stainless steel surfaces. The results of colorimetric assays, indicating significant differences between the amounts of neutral sugars present in these biofilms, were supported by gas chromatographic (GC)-mass spectrophotometric and GC-flame ionisation detection analyses. Neutral sugars in biofilms grown on mild steel surfaces were identified and quantified, revealing glucose as a major carbohydrate followed by mannose and galactose in all types of biofilm. Extracellular polymeric substances (EPS) precipitated from bacterial cultures grown with and without steel surfaces were also analysed for their carbohydrate content. The influence of the surfaces present in the cultures on the amount and type of sugars released into the bulk phase was established. There was significantly more carbohydrate in EPS harvested from pure and mixed cultures of D. desulfuricans incubated mild and stainless steel coupons than in EPS obtained from coupon-free cultures. No significant difference in sugar quantities was observed in EPS precipitated from cultures of P. fluorescens grown under different conditions (absence or presence of steel surfaces). The main carbohydrates identified in all types of EPS samples were mannose, glucose and galactose in order of prevalence.en_US
dc.titleExtracellular polysaccharides from desulfovibrio desulfuricans and pseudomonas fluorescens in the presence of mild and stainless steelen_US
mus.citation.journaltitleApplied Microbiology and Biotechnologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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