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dc.contributor.authorMueller, Robert Franz
dc.contributor.authorCharacklis, William G.
dc.contributor.authorJones, Warren L.
dc.contributor.authorSpears, J.
dc.identifier.citationMueller, R.F., W.G. Characklis, W.L. Jones and J.T. Sears, "Characterization of initial events in bacterial surface colonization by two pseudomonas species using image analysis," Biotechnology and Bioengineering, 39(11): 1161-1170 (1992).en_US
dc.description.abstractThe processes leading to bacterial colonization on solidwater interfaces are adsorption, desorption, growth, and erosion. These processes have been measured individually in situ in a flowing system in real time using image analysis. Four different substrata (copper, silicon, 316 stainless-steel and glass) and 2 different bacterial species (Pseudomonas aeruginosa and Pseudomonas fluorescens) were used in the experiments. The flow was laminar (Re = 1.4) and the shear stress was kept constant during all experiments at 0.75 N m−2. The surface roughness varied among the substrata from 0.002 μm (for silicon) to 0.015 μm (for copper). Surface free energies varied from 25.1 dynes cm−1 for silicon to 31.2 dynes cm−1 for copper. Cell curface hydrophobicity, reported as hydrocarbon partitioning values, ranged from 0.67 for Ps. fluorescensto 0.97 for Ps. aeruginosa. The adsorption rate coefficient varried by as much as a factor of 10 among the combinations of bacterial strain and substratum material, and was positively correlated with surface free energy, the surface roughness of the substratum, and the hydrophobicity of the cells. The probability of desorption decreased with increasing surface free energy and surface roughness of the substratum. Cell growth was inhibited on copper, but replication of cells overlying an initial cell layer was observed with increased exposure time to the cell-containing bulk water. A mathematical model describing cell accumulation on a substratum is presented.en_US
dc.titleCharacterization of initial events in bacterial surface colonization by two pseudomonas species using image analysisen_US
mus.citation.journaltitleBiotechnology and Bioengineeringen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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