Show simple item record

dc.contributor.authorDrury, William J.
dc.contributor.authorStewart, Philip S.
dc.contributor.authorCharacklis, William G.
dc.date.accessioned2017-11-15T22:19:39Z
dc.date.available2017-11-15T22:19:39Z
dc.date.issued1993-06
dc.identifier.citationDrury, W.J., P.S. Stewart, and W.G. Characklis, "Transport of 1-μm latex particles in pseudomonas aeruginosa biofilms," Biotechnology and Bioengineering, 42(1):111-117 (1993).en_US
dc.identifier.issn0006-3592
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14016
dc.description.abstractFluorescent latex microbeads added to a Pseudomonas aeruginosa biofilm as tracers of particle movement penetrated the biofilm and remained in it much longer than predicted by a model of advective displacement due to cell growth. Beads with a nominal diameter of 1 μm that were added in the bulk fluid became distributed throughout the biofilm depth. Some microbeads penetrated to the substratum within the 24-h bead addition period. The biofilms had a mean thickness of approximately 34 μm but have been previously shown to be quite rough. Measured rates of bead release from the biofilm corresponded to first order time coefficients of 0.01–0.03 h−1. These bead release rates were approximately an order of magnitude less than the predicted time scale of advective transport, which is just the experimentally measured specific cellular growth rate of 0.15 h−1. Computer simulations of bead transport using the biofilm model BIOSIM were compared with bead release rate data and with bead position distributions within the biofilm as determined by microscopic examination of thin cross sections of embedded biofilm. The model predicted much faster release of beads from the biofilm than actually occurred. It is hypothesized that both the ability of beads to penetrate the biofilm and the unexpectedly low advective displacement velocity of particles in the biofilm were due to the rough nature of the biofilm.en_US
dc.titleTransport of 1-μm latex particles in pseudomonas aeruginosa biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage111en_US
mus.citation.extentlastpage117en_US
mus.citation.issue1en_US
mus.citation.journaltitleBiotechnology and Bioengineeringen_US
mus.citation.volume42en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1002/bit.260420115en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record


MSU uses DSpace software, copyright © 2002-2017  Duraspace. For library collections that are not accessible, we are committed to providing reasonable accommodations and timely access to users with disabilities. For assistance, please submit an accessibility request for library material.