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dc.contributor.authorStewart, Philip S.
dc.date.accessioned2017-12-14T21:24:12Z
dc.date.available2017-12-14T21:24:12Z
dc.date.issued1994-05
dc.identifier.citationStewart, P.S., "Biofilm accumulation model that predicts antibiotic resistance of pseudomonas aeruginosa biofilms," Antimicrobial Agents and Chemotherapy, 38(5):1052-1058 (May 1994).en_US
dc.identifier.issn0066-4804
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14084
dc.description.abstractA computer model of biofilm dynamics was adapted to incorporate the activity of an antimicrobial agent on bacterial biofilm. The model was used to evaluate the plausibility of two mechanisms of biofilm antibiotic resistance by qualitative comparison with data from a well-characterized experimental system (H. Anwar, J. L. Strap, and J. W. Costerton, Antimicrob. Agents Chemother. 36:1208-1214, 1992). The two mechanisms involved either depletion of the antibiotic by reaction with biomass or physiological resistance due to reduced bacterial growth rates in the biofilm. Both mechanisms predicted the experimentally observed resistance of 7-day-old Pseudomonas aeruginosa biofilms compared with that of 2-day-old ones. A version of the model that incorporated growth rate-dependent killing predicted reduced susceptibility of thicker biofilms because oxygen was exhausted within these biofilms, leading to very slow growth in part of the biofilm. A version of the model that incorporated a destructive reaction of the antibiotic with biomass likewise accounted for the relative resistance of thicker biofilms. Resistance in this latter case was due to depletion of the antibiotic in the bulk fluid rather than development of a gradient in the antibiotic concentration within the biofilm. The modeling results predicted differences between the two cases, such as in the survival profiles within the biofilm, that could permit these resistance mechanisms to be experimentally distinguished.en_US
dc.titleBiofilm accumulation model that predicts antibiotic resistance of pseudomonas aeruginosa biofilmsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1052en_US
mus.citation.extentlastpage1058en_US
mus.citation.issue5en_US
mus.citation.journaltitleAntimicrobial Agents and Chemotherapyen_US
mus.citation.volume38en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1128/aac.38.5.1052en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US


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