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dc.contributor.authorYu, Feipeng Philip
dc.contributor.authorMcFeters, Gordon A.
dc.date.accessioned2017-12-14T21:47:15Z
dc.date.available2017-12-14T21:47:15Z
dc.date.issued1994-06
dc.identifier.citationYu, F.P. and G.A. McFeters, "Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes," Journal of Microbiological Methods, 20(1):1-10 (1994).en_US
dc.identifier.issn0167-7012
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14086
dc.description.abstractTwo rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox indicator which can be reduced by respiring bacteria to fluorescent CTC-formazan crystals. Rh 123 is incorporated into bacteria with respect to cellular proton motive force. The intracellular accumulation of these fluorescent dyes can be determined using epifluorescence microscopy. The results obtained with these two fluorescent probes in situ were compared to the plate count (PC) and in situ direct viable count (DVC) methods. Viable cell densities within biofilms determined by the three in situ methods were comparable and always showed approximately 2-fold higher values that those obtained with the PC method. As an additional advantage, the results were observed after 2 h, which was shorter than the 4 h incubation time required for the DVC method and 24 h for colony formation. The results indicate that staining with CTC and Rh 123 provides rapid information regarding cell numbers and physiological activities of bacteria within biofilms.en_US
dc.titleRapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage1en_US
mus.citation.extentlastpage10en_US
mus.citation.issue1en_US
mus.citation.journaltitleJournal of Microbiological Methodsen_US
mus.citation.volume20en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1016/0167-7012(94)90058-2en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage5en_US


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