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dc.contributor.authorRice, A. R.
dc.contributor.authorHamilton, Martin A.
dc.contributor.authorCamper, Anne K.
dc.date.accessioned2018-01-17T22:43:41Z
dc.date.available2018-01-17T22:43:41Z
dc.date.issued2000-07
dc.identifier.citationRice, A.R., M.A. Hamilton, and A.K. Camper, "Apparent Surface Associated Lag Time in Growth of Primary Biofilm Cells,"" 40(1):8-15 (2000).en_US
dc.identifier.issn0095-3628
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14139
dc.description.abstractThe ability of microorganisms to form biofilms has been well documented. Bacterial cells make a transition from a planktonic state to a sessile state, replicate, and subsequently populate a surface. In this study, organisms that initially colonize a ``clean'' surface are referred to as ``primary'' biofilm cells. The progeny of the first generation of sessile cells are known as ``secondary'' biofilm cells. This study examined the growth of planktonic, primary, and secondary biofilm cells of a green fluorescent protein producing (GFP+) Pseudomonas aeruginosa PA01. Biofilm experiments were performed in a parallel plate flow cell reactor with a glass substratum. Individual cells were tracked over time using a confocal scanning laser microscope (CSLM). Primary cells experience a lag in their growth that may be attributed to adapting to a sessile environment or undergoing a phenotypic change. This is referred to as a surface associated lag time. Planktonic and secondary biofilm cells both grew at a faster rate than the primary biofilm cells under the same nutrient conditions.en_US
dc.titleApparent surface associated lag time in growth of primary biofilm cellsen_US
dc.typeArticleen_US
mus.citation.extentfirstpage8en_US
mus.citation.extentlastpage15en_US
mus.citation.issue1en_US
mus.citation.journaltitleMicrobial Ecologyen_US
mus.citation.volume40en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/s002480000011en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage3en_US


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