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dc.contributor.authorHuang, Ching-Tsan
dc.contributor.authorPeretti, Steven W.
dc.contributor.authorBryers, James D.
dc.date.accessioned2018-01-19T17:54:11Z
dc.date.available2018-01-19T17:54:11Z
dc.date.issued1994-09
dc.identifier.citationHuang, C.-T., S.W. Peretti, and J.D. Bryers, "Effects of inducer levels on a recombinant bacterial biofilm formation and gene expression," Biotechnology Letters, 16(9):903-908 (1994).en_US
dc.identifier.issn0141-5492
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14178
dc.description.abstractA segregationally stable host-plasmid system, E. coli DH5α (pTKW106), was used to study the effect of induction on the accumulation rate of cells and gene expression in biofilm cultures. Isopropyl β-D-thiogalactoside (IPTG) was used to induce the expression of β-galactosidase from the plasmid. The biofilm cell net accumulation rates decreased with increasing induction levels. At 0.17 and 0.34 mM of IPTG, the biofilm cell net accumulation rates ranged between 17 and 30% when compared to the uninduced case. At 0.51 mM of IPTG, the biofilm cell density never increased. At 0.17 and 0.34 mM of IPTG, β-galactosidase contents reached maxima 36 hours after induction with both amounts representing about 7.5% of total protein. At 0.51 mM of IPTG, β-galactosidase production reached its maximum, about 16% of total protein, 48 hours after induction. The β-galactosidase mRNA synthesis rates increased with increasing inducer levels. Maximum β-galactosidase mRNA synthesis rates were reached 36 hours after induction for each IPTG concentration.en_US
dc.titleEffects of inducer levels on a recombinant bacterial biofilm formation and gene expressionen_US
dc.typeArticleen_US
mus.citation.extentfirstpage903en_US
mus.citation.extentlastpage908en_US
mus.citation.issue9en_US
mus.citation.journaltitleBiotechnology Lettersen_US
mus.citation.volume16en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1007/bf00128622en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage4en_US


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