Estimation of cellular and extracellular carbon contents in sulfate-reducing bacteria biofilms by lipopolysaccharide assay and epifluorescence microscopic techni
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1994-11
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Abstract
Measurement of cellular and extracellular carbon contents of sulfate-reducing bacteria (SRB) is essential and important in studies of the role of SRB in corrosion and biofouling. An epifluorescence (EPI) microscopic technique and a lipopolysaccharide (LPS) assay were used to quantify cellular and extracellular carbon contents in Desulfovibrio desulfuricans biofilms. The average contents of lipopolysaccharide (LPS) and cellular carbon were 7.3 ± 2.8 (fg LPS) cell−1 and 39.9 ± 9.9 (fg cellular-C) cell−1, respectively, in a D. desulfuricans chemostat culture. A ratio of cellular carbon content to LPS content was 6.5 ± 2.8, and was used to estimate cellular carbon contents in a D. desulfuricans biofilm. The LPS and EPI methods gave comparable results for suspended samples, but not for biofilm samples.
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Okabe, S., P.H. Nielsen, W.L. Jones, and W.G. Characklis, "Estimation of cellular and extracellular carbon contents in sulfate-reducing bacteria biofilms by lipopolysaccharide assay and epifluorescence microscopic techni," Water Research, 28(11):2263-2266 (1994).