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dc.contributor.authorBryers, James D.
dc.contributor.authorHuang, Ching-Tsan
dc.identifier.citationBryers, J.D. and C.-T. Huang, "Recombinant Plasmid Retention and Expression in Bacterial Biofilm Cultures," Water Sci. Technol., 31(1):105-115 (1995).en_US
dc.description.abstractAny exposure of plasmid recombinant microorganisms to an open system environment, either inadvertently or intentionally, mandates research into those fundamental organism:plasmid processes that influence plasmid retention, transfer and expression. In open environmental systems a majority of the microbial activity occurs associated with an interface, within thin biological layers consisting of the cells and their insoluble extracellular polymer, layers known as biofilms. Thus any study regarding the fate of recombinant DNA sequences in an open system must consider processes that affect plasmid retention and expression in a biofilm culture. Biofilm cultures were cultivated in a parallel-plate flow cell reactor using E. coli DH5α which contained a recombinant plasmid with a plasmid stability factor, parB, (pTKW106) or without (pMJR1750). Using β-galactosidase as inducible reporter protein, plasmid retention and gene expression of pMJR1750 and pTKW106, in suspended versus biofilm cultures, were studied under different carbo to nitrogen ratios and plasmid induction levels. Recombinant biofilm formation under these environmental conditions was also investigated. Biofilm net accumulation rate of E. coli DH5α (pTKW106) decreases with increasing induction levels. The β-galactosidase production and ratios of β-galactosidase to total protein increase with increasing induction levels. Synthesis rates of total RNA, β-galactosidase mRNA and rRNA in biofilm cultures of E. coli DH5α (pTKW106) increase after induction by IPTG.en_US
dc.titleRecombinant plasmid retention and expression in bacterial biofilm culturesen_US
mus.citation.journaltitleWater Science and Technologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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