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dc.contributor.authorSrinivasan, Rohini
dc.contributor.authorStewart, Philip S.
dc.contributor.authorGriebe, Thomas
dc.contributor.authorChen, Ching-I
dc.contributor.authorXu, Xiaoming
dc.date.accessioned2018-01-30T16:22:01Z
dc.date.available2018-01-30T16:22:01Z
dc.date.issued1995-06
dc.identifier.citationSrinivasan, R., P.S. Stewart, T. Griebe, C.-I. Chen, and X. Xu, "Biofilm Parameters Influencing Biocide Efficacy," Biotechnology and Bioengineering, 46:553-560 (1995).en_US
dc.identifier.issn0006-3592
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14221
dc.description.abstractThe influence of biofilm areal cell density, species composition, and the presence of abiotic particles on the disinfection and removal of bacterial biofilms by monochloramine was investigated. Mono- and binary population biofilms of Pseudomonas aeruginosa and Klebsiella pneumoniae were grown on stainless-steel slides in a continuous flow annular reactor. Biofilms were treated in the reactor with a pulse/step dose of 4 mg/L monochloramine for 2 h. Biofilm samples were disaggregated and assayed for colony formation on R2A agar and for total cell numbers by acridine orange direct counts. These data were used to determine apparent first order rate coefficients for the processes of disinfection and detachment. Disinfection rate coefficients exceeded detachment rate coefficients by as much as an order of magnitude and the two coefficients were poorly correlated (r = 0.272). The overall decay rate coefficient (disinfection plus detachment) depended strongly on the initial biofilm areal cell density. It displayed a parabolic dependence on cell density with a maximum near 108 cfu/cm2. This result points to multiple factors influencing biofilm susceptibility to antimicrobial challenge. Decay rates of K. pneumoniae measured in binary population biofilms were comparable with those measured in monopopulation biofilms (p = 0.61). P. aeruginosa decayed more slowly in biofilsm dominated by K. pneumoniae (p = 0.028), indicating some interaction between species. The presence of kaolin and calcium carbonate particles in the biofilm reduced disinfection efficacy. © 1995 John Wiley & Sons, Inc.en_US
dc.titleBiofilm parameters influencing biocide efficacyen_US
dc.typeArticleen_US
mus.citation.extentfirstpage553en_US
mus.citation.extentlastpage560en_US
mus.citation.issue6en_US
mus.citation.journaltitleBiotechnology and Bioengineeringen_US
mus.citation.volume46en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1002/bit.260460608en_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.data.thumbpage6en_US
mus.contributor.orcidStewart, Philip S.|0000-0001-7773-8570en_US


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