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dc.contributor.authorde Beer, Dirk
dc.contributor.authorStoodley, Paul
dc.contributor.authorLewandowski, Zbigniew
dc.date.accessioned2018-02-01T22:30:32Z
dc.date.available2018-02-01T22:30:32Z
dc.date.issued1997-01
dc.identifier.citationde Beer, D. P. Stoodley, and Z. Lewandowski, “Measurement of Local Diffusion Coefficients in Biofilms by Microinjection and Confocal Microscopy,” Biotechnology and Bioengineering, 53(2):151-158 (1997)en_US
dc.identifier.issn0006-3592
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14288
dc.description.abstractA new technique for the determination of local diffusion coefficients in biofilms is described. It is based on the microinjection of fluorescent dyes and quantitative analysis of the subsequent plume formation using confocal laser microscopy. The diffusion coefficients of fluorescein (MW 332), TRITC-IgG (MW 150000) and phycoerythrin (MW 240000) were measured in the cell clusters and interstitial voids of a heterogeneous biofilm. The diffusivities measured in the voids were close to the theoretical values in water. Fluorescein had the same diffusivity in cell clusters, voids, and sterile medium. TRITC-IgG did not diffuse in cell clusters, presumably due to binding to the cell cluster matrix. After treatment of the biofilm with bovine serum albumin, binding capacity decreased and the diffusion coefficient could be measured. The diffusivity of phycoerythrin in cell clusters was impeded by 41%, compared to interstitial voids. From the diffusion data of phycoerythrin it was further calculated that the cell cluster matrix had the characteristics of a gel with 0.6 nm thick fibers and pore diameters of 80 nm. © 1997 John Wiley & Sons, Inc.en_US
dc.titleMeasurement of local diffusion coefficients in biofilms by microinjection and confocal microscopyen_US
dc.typeArticleen_US
mus.citation.extentfirstpage151en_US
mus.citation.extentlastpage158en_US
mus.citation.issue2en_US
mus.citation.journaltitleBiotechnology and Bioengineeringen_US
mus.citation.volume53en_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.identifier.doi10.1002/(sici)1097-0290(19970120)53:2<151::aid-bit4>3.0.co;2-nen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.relation.researchgroupEnergy Research Institute.en_US
mus.data.thumbpage5en_US
mus.contributor.orcidde Beer, Dirk|0000-0001-5274-1781en_US
mus.contributor.orcidStoodley, Paul|0000-0001-6069-273Xen_US


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