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dc.contributor.authorBryers, James D.
dc.contributor.authorSharp, Robert R.
dc.identifier.citationBryers, J.D. and R.R. Sharp, “Retention and Expression of Recombinant Plasmids in Suspended and Biofilm-Bound Bacteria Degrading Trichloroethene (TCE), Water Science and Technology, 36(10):1-8 (1997).en_US
dc.description.abstractExposure of plasmid recombinant microorganisms to an open environment, either inadvertently or intentionally, requires research into those fundamental processes that govern plasmid retention, transfer and expression. In the open environment, a majority of the microbial activity occurs associated with an interface, within thin biological layers consisting of cells and their insoluble extracellular polymer, layers known as biofilms. Current toxic wastewater or wastegas treatment reactors exploit bacterial biofilm systems for certain system operating advantages. Using recombinant bacteria within a biofilm reactor to degrade xenobiotic wastes requires finding a suitable host to harbor and express the desired plasmid phenotype. Suitable host characteristics include: the ability to produce copious amounts of biofilm, resistance to waste-related injury and toxicity, and the ability to retain and express the desired plasmid during long term operation. This paper reports on a laboratory evaluation of factors governing plasmid retention and the expression of trichloroethene (TCE) degradative capacity in both suspended and biofilm cultures.en_US
dc.titleRetention and expression of recombinant plasmids in suspended and biofilm-bound bacteria degrading trichloroethene (tce)en_US
mus.citation.journaltitleWater Science and Technologyen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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