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dc.contributor.authorScheuerman, Teresa R.
dc.contributor.authorCamper, Anne K.
dc.contributor.authorHamilton, Martin A.
dc.identifier.citationScheuerman, T.R., A.K. Camper and M.A. Hamilton, "Effects of substratum topography on bacterial adhesion," Journal of Colloid and Interface Science," 208:23-33 (1998).en_US
dc.description.abstractThe effect of substratum topography on bacterial surface colonization was studied using a chemically homogeneous silicon coupon. “Grooves” 10 μm deep and 10, 20, 30, and 40 μm wide were etched on the coupon perpendicular to the direction of flow. Flow (Re = 5.5) of a bacterial suspension (108cells/ml) was directed through a parallel plate flow chamber inverted on a confocal microscope. Images were collected in real time to obtain rate and endpoint colonization data for each of three strains of bacteria:Pseudomonas aeruginosaand motile and nonmotilePseudomonas fluorescens.A higher velocity experiment (Re = 16.6) and an abiotic control using hydrophilic, negatively charged microspheres were also performed. Using a colloidal deposition expression, the initial rates of attachment were compared.P. aeruginosaattached at a higher rate thanP. fluorescensmot+ which attached at a higher rate thanP. fluorescensmot−. For all bacteria the rate was independent of groove size and was greatest on the downstream edges of the grooves. Only the motile organisms were found in the bottoms of the grooves. A higher fluid velocity resulted in an increase in the initial rate of attachment. In contrast, there was no adhesion of the beads. Attachment of the bacteria appears to be predominated by transport from the bulk phase to the substratum.en_US
dc.titleEffects of substratum topography on bacterial adhesionen_US
mus.citation.journaltitleJournal of Colloid and Interface Scienceen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US

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