Conformational regulation of CRISPR-associated nucleases
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Adaptive immune systems in bacteria and archaea rely on small CRISPR-derived RNAs (crRNAs) to guide specialized nucleases to foreign nucleic acids. The activation of these nucleases is controlled by a series of molecular checkpoints that ensure precise cleavage of nucleic acid targets, while minimizing toxic off-target cleavage events. In this review, we highlight recent advances in understanding regulatory mechanisms responsible for controlling the activation of these nucleases and identify emerging regulatory themes conserved across diverse CRISPR systems.
Jackson, Ryan N. , Paul Bg van Erp, Samuel H. Sternberg, and Blake Wiedenheft. "Conformational regulation of CRISPR-associated nucleases." Current Opinion in Microbiology 37 (June 2017): 110-119. DOI: 10.1016/j.mib.2017.05.010.