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dc.contributor.authorHamerly, Timothy
dc.contributor.authorEverett, Jake A.
dc.contributor.authorParis, Nina
dc.contributor.authorFisher, Steve T.
dc.contributor.authorKarunamurthy, Arivarasan
dc.contributor.authorJames, Garth A.
dc.contributor.authorRumbaugh, Kendra P.
dc.contributor.authorRhoads, Daniel D.
dc.contributor.authorBothner, Brian
dc.identifier.citationHamerly T, JA Everett, N Paris, ST Fisher, A Karunamurthy, GA James, KP Rumbaugh, DD Rhoads, B Bothner, “Detection of Pseudomonas aeruginosa biomarkers from thermally injured mice in situ using imaging mass spectrometry,” Analytical Biochemistry, December 2017; 539:144-148. doi: 10.1016/j.ab.2017.10.012.en_US
dc.description.abstractMonitoring patients with burn wounds for infection is standard practice because failure to rapidly and specifically identify a pathogen can result in poor clinical outcomes, including death. Therefore, a method that facilitates detection and identification of pathogens in situ within minutes of biopsy would be a significant benefit to clinicians. Mass spectrometry is rapidly becoming a standard tool in clinical settings, capable of identifying specific pathogens from complex samples. Imaging mass spectrometry (IMS) expands the information content by enabling spatial resolution of biomarkers in tissue samples as in histology, without the need for specific stains/antibodies. Herein, a murine model of thermal injury was used to study infection of burn tissue by Pseudomonas aeruginosa. This is the first use of IMS to detect P. aeruginosa infection in situ from thermally injured tissue. Multiple molecular features could be spatially resolved to infected or uninfected tissue. This demonstrates the potential use of IMS in a clinical setting to aid doctors in identifying both presence and species of pathogens in tissue.en_US
dc.titleDetection of Pseudomonas aeruginosa biomarkers from thermally injured mice in situ using imaging mass spectrometryen_US
mus.citation.journaltitleAnalytical Biochemistryen_US
mus.identifier.categoryEngineering & Computer Scienceen_US
mus.relation.collegeCollege of Engineeringen_US
mus.relation.departmentCenter for Biofilm Engineering.en_US
mus.relation.departmentChemical & Biological Engineering.en_US
mus.relation.departmentChemical Engineering.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.relation.researchgroupCenter for Biofilm Engineering.en_US
mus.contributor.orcidBothner, Brian|0000-0003-1295-9609en_US
mus.contributor.orcidHamerly, Timothy|0000-0002-8670-3721en_US

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