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dc.contributor.authorMartin, John M.
dc.contributor.authorHogg, Andrew C.
dc.contributor.authorWebster, Richard W.
dc.contributor.authorGiroux, Michael J.
dc.identifier.citationMartin, John M. , Andrew C. Hogg, Richard W. Webster, and Michael J. Giroux. "Creation and Characterization of a Double Null Puroindoline Genotype in Spring Wheat." Cereal Chemistry 94, no. 5 (September 2017): 805-810. DOI: 10.1094/CCHEM-04-17-0071-RW.en_US
dc.description.abstractWheat (Triticum aestivum L.) grain hardness is controlled by the Ha locus, which is composed of two closely linked genes, Puroindoline a (Pina) and Puroindoline b (Pinb). Hard grain results from mutations in either of the Pin genes. Previous results have shown that the Pina-D1b (Pina null) allele has harder grain than other naturally occurring Pin alleles. Our goal was to create, identify, and characterize a double null Pin genotype by identifying a Pinb null mutation in a variety carrying the Pina-D1l null allele. Seeds of Fortuna, which has a premature stop codon in Pina, were treated with ethyl methanesulfonate. Two premature stop codon mutations were identified in Pinb using direct sequencing. The double null Pin haplotype was characterized after backcrossing to the parent variety Fortuna to create Pina null populations segregating for the presence of Pinb. The double null group was 6 units harder than the single null with no difference in other kernel characteristics. The milling characteristics differed between the two classes; the double null class had less break flour with a greater fraction of large and a smaller fraction of small flour particles compared with the single null class. Neither water absorption nor loaf volume was impacted by the change in grain hardness; however, Na2CO3 tests indicated greater starch damage in the double nulls. The double null Pin genotype may find a niche in hard wheat products for which flours with larger particle size are desired.en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.titleCreation and Characterization of a Double Null Puroindoline Genotype in Spring Wheaten_US
mus.citation.journaltitleCereal Chemistryen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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