Show simple item record

dc.contributor.advisorChairperson, Graduate Committee: Andreas Fischeren
dc.contributor.authorMangel, Dylan J. L.en
dc.date.accessioned2018-12-05T19:30:11Z
dc.date.available2018-12-05T19:30:11Z
dc.date.issued2018en
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14690
dc.description.abstractCereal grain protein concentration (GPC) is an important quality parameter, with high GPC desirable when grain is used for food or feed, while low (typically below 12- 13%) GPC is needed when barley grain is used for malting. A major QTL controlling grain protein has previously been identified on barley chromosome 6H. Based on the comparison with a co-linear region on wheat chromosome 6B, the functional status of a gene coding for a NAC transcription factor (HvNAM-1) is responsible for controlling whole-plant senescence, nutrient remobilization from leaves to developing grains, and grain protein concentration. In this context, the purpose of this study was the analysis of the influence of a low- vs. a high-grain protein allele at this locus on barley agronomic and malting quality parameters in different genetic backgrounds. Integration of the low-protein allele stably lowered GPC by 1.6-1.8%, in both years and across all combinations of genetic backgrounds and management practices. Lines with the low-protein allele matured 1.4 to 2.5 days later, dependent on management, and had stably lower malt protein and diastatic power. Effects on additional agronomic and malt quality parameters including yield, test weight, percentage of plump kernels, free amino nitrogen and alpha-amylase activity were subtler and depended on the genetic background tested. Our data also provide important information on the influence of soil N and water availability on malt quality characteristics. Overall, use of the chromosome 6H low-grain protein allele stably lowered grain and malt protein levels without important negative influences on any of the tested agronomic and malt quality parameters, indicating its usefulness in the development of new malting barley germplasm. The studied chromosome 6H GPC QTL also controls expression of a gene coding for a glycine-rich RNA-binding protein (HvGR-RBP1) that may improve drought tolerance. Stomatal conductance and canopy thermal imaging assays did not show consistent effects of the allelic state of the GPC QTL on stomatal control. However, our data showed important correlations between stomatal conductance, canopy temperature depression and agronomic parameters including yield, demonstrating the value of the performed assays for variety selection and breeding.en
dc.language.isoenen
dc.publisherMontana State University - Bozeman, College of Agricultureen
dc.subject.lcshBarley.en
dc.subject.lcshPlant proteins.en
dc.subject.lcshGenomics.en
dc.subject.lcshIrrigation.en
dc.subject.lcshFertilizers.en
dc.subject.lcshMutation (Biology).en
dc.titleEffects of a barley chromosome 6H grain protein QTL on agronomic traits, malt quality traits, and stomatal control under two irrigation and nitrogen fertilization regimesen
dc.typeThesisen
dc.rights.holderCopyright 2018 by Dylan J. L. Mangelen
thesis.degree.committeemembersMembers, Graduate Committee: Andreas Fischer (chairperson); Jamie Sherman; Jack Martin.en
thesis.degree.departmentPlant Sciences & Plant Pathology.en
thesis.degree.genreThesisen
thesis.degree.nameMSen
thesis.format.extentfirstpage1en
thesis.format.extentlastpage95en
mus.data.thumbpage76en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record