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dc.contributor.authorZeng, Huawei
dc.contributor.authorIshaq, Suzanne L.
dc.contributor.authorLiu, Zhenhua
dc.contributor.authorBukowsky, Michael R.
dc.date.accessioned2018-09-10T21:28:22Z
dc.date.available2018-09-10T21:28:22Z
dc.date.issued2018-04
dc.identifier.citationZeng, Huawei, Suzanne L. Ishaq, Zhenhua Liu, and Michael R. Bukowski. "Colonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat diet." Journal of Nutritional Biochemistry 54 (April 2018): 18-27. DOI: 10.1016/j.jnutbio.2017.11.001.en_US
dc.identifier.issn0955-2863
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14742
dc.description.abstractThe increasing worldwide incidence of colon cancer has been linked to obesity and consumption of a high-fat western diet. To test the hypothesis that a high fat diet (HFD) promotes colonic aberrant crypt (AC) formation in a manner associated with gut bacterial dysbiosis, we examined the susceptibility to azoxymethane (AOM)-induced colonic AC and microbiome composition in C57/BL6 mice fed a modified AIN93G diet (AIN, 16% fat, energy) or a HFD (45% fat, energy) for 14 weeks. Mice receiving the HFD exhibited increased plasma leptin, body weight, body fat composition and inflammatory cell infiltration in the ileum compared with those in the AIN group. Consistent with the gut inflammatory phenotype, we observed an increase in colonic AC, plasma interleukin 6 (IL6), tumor necrosis factor α (TNF α), monocyte chemoattractant protein 1 (MCP1), and inducible nitric oxide synthase (iNOS) in the ileum of the HFD-AOM group compared with the AIN-AOM group. Although the HFD and AIN groups did not differ in bacterial species number, the HFD and AIN diets resulted in different bacterial community structures in the colon. The abundance of certain short chain fatty acid (SCFA) producing bacteria (e.g., Barnesiella) and fecal SCFA (e.g., acetic acid) content were lower in the HFD-AOM group compared with the AIN and AIN-AOM groups. Furthermore, we identified a high abundance of Anaeroplasma bacteria, an opportunistic pathogen in the HFD-AOM group. Collectively, we demonstrate that a HFD promotes AC formation concurrent with an increase of opportunistic pathogenic bacteria in the colon of C57BL/6 mice.en_US
dc.description.sponsorshipUS Department of Agricultureen_US
dc.language.isoenen_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.titleColonic aberrant crypt formation accompanies an increase of opportunistic pathogenic bacteria in C57BL/6 mice fed a high-fat dieten_US
dc.typeArticleen_US
mus.citation.extentfirstpage18en_US
mus.citation.extentlastpage27en_US
mus.citation.journaltitleJournal of Nutritional Biochemistryen_US
mus.citation.volume54en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.1016/j.jnutbio.2017.11.001en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage5en_US
mus.contributor.orcidIshaq, Suzanne L.|0000-0002-2615-8055en_US


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