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dc.contributor.authorIshaq, S. L.
dc.contributor.authorYeoman, Carl J.
dc.contributor.authorWhitney, T. R.
dc.date.accessioned2018-09-14T21:25:15Z
dc.date.available2018-09-14T21:25:15Z
dc.date.issued2018-12
dc.identifier.citationIshaq, Suzanne L., Carl J. Yeoman, and T. R. Whitney. "Ground Juniperus pinchotii and urea in supplements fed to Rambouillet ewe lambs Part 2: Ewe lamb rumen microbial communities." Journal of Animal Science 95, no. 10 (December 2017): 4587-4599. DOI: 10.2527/jas2017.1731.en_US
dc.identifier.issn0021-8812
dc.identifier.urihttps://scholarworks.montana.edu/xmlui/handle/1/14842
dc.description.abstractThis study evaluated effects of ground redberry juniper (Juniperus pinchotii) and urea in dried distillers grains with solubles-based supplements fed to Rambouillet ewe lambs (n = 48) on rumen physiological parameters and bacterial diversity. In a randomized study (40 d), individually-penned lambs were fed ad libitum ground sorghum-sudangrass hay and of 1 of 8 supplements (6 lambs/treatment; 533 g/d; as-fed basis) in a 4 x 2 factorial design with 4 concentrations of ground juniper (15%, 30%, 45%, or 60% of DM) and 2 levels of urea (1% or 3% of DM). Increasing juniper resulted in minor changes in microbial β-diversity (PERMANOVA, pseudo F = 1.33, P = 0.04); however, concentrations of urea did not show detectable broad-scale differences at phylum, family, or genus levels according to ANOSIM (P > 0.05), AMOVA (P > 0.10), and PERMANOVA (P > 0.05). Linear discriminant analysis indicated some genera were specific to certain dietary treatments (P < 0.05), though none of these genera were present in high abundance; high concentrations of juniper were associated with Moraxella and Streptococcus, low concentrations of urea were associated with Fretibacterium, and high concentrations of urea were associated with Oribacterium and Pyramidobacter. Prevotella were decreased by juniper and urea. Ruminococcus, Butyrivibrio, and Succiniclasticum increased with juniper and were positively correlated (Spearman\'s, P < 0.05) with each other but not to rumen factors, suggesting a symbiotic interaction. Overall, there was not a juniper x urea interaction for total VFA, VFA by concentration or percent total, pH, or ammonia (P > 0.29). When considering only percent inclusion of juniper, ruminal pH and proportion of acetic acid linearly increased (P < 0.001) and percentage of butyric acid linearly decreased (P = 0.009). Lamb ADG and G:F were positively correlated with Prevotella (Spearman\'s, P < 0.05) and negatively correlated with Synergistaceae, the BS5 group, and Lentisphaerae. Firmicutes were negatively correlated with serum urea nitrogen, ammonia, total VFA, total acetate, and total propionate. Overall, modest differences in bacterial diversity among treatments occurred in the abundance or evenness of several OTUs, but there was not a significant difference in OTU richness. As diversity was largely unchanged, the reduction in ADG and lowerend BW was likely due to reduced DMI rather than a reduction in microbial fermentative ability.en_US
dc.description.sponsorshipUSDA National Institute of Food and Agriculture Hatch Project #205866; Food and Fibers Research Grant Program administered by the Texas Department of Agricultureen_US
dc.language.isoenen_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en_US
dc.titleGround Juniperus pinchotii and urea in supplements fed to Rambouillet ewe lambs Part 2: Ewe lamb rumen microbial communitiesen_US
dc.typeArticleen_US
mus.citation.extentfirstpage4587en_US
mus.citation.extentlastpage4599en_US
mus.citation.issue10en_US
mus.citation.journaltitleJournal of Animal Scienceen_US
mus.citation.volume95en_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.identifier.doi10.2527/jas2017.1731en_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentAnimal & Range Sciences.en_US
mus.relation.universityMontana State University - Bozemanen_US
mus.data.thumbpage9en_US


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