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dc.contributor.authorBibi, Fehmida
dc.contributor.authorStrobel, Gary A.
dc.contributor.authorNaseer, Muhammad I.
dc.contributor.authorYasir, Muhammad
dc.contributor.authorKhalaf Al-Ghamdi, Ahmed A.
dc.contributor.authorAzhar, Esam I.
dc.identifier.citationBibi, Fehmida, Gary Allan Strobel, Muhammad Imran Naseer, Muhammad Yasir, Ahmed Abdullah Khalaf Al-Ghamdi, and Esam Ibrahim Azhar. "Halophytes-associated endophytic and rhizospheric bacteria: diversity, antagonism and metabolite production." Biocontrol Science and Technology 28, no. 2 (February 2018): 192-213. DOI: 10.1080/09583157.2018.1434868.en_US
dc.description.abstractIn Saudi Arabia, halophytes occupy tidal and intertidal forest ecosystems. They and their associated microflora have immense potential to yield novel and important useful natural products. Three halophytes (Avicennia marina, Halocnemum strobilaceum, Zygophyllum qatarense) were targeted for the isolation and identification of populations of endophytic and rhizospheric bacteria having antimicrobial potential. A total 554 bacterial isolates were initially screened against oomycetes fungal pathogens, Phytophthora capsici and Pythium ultimum. Of these, only 57 rhizospheric and endophytic bacteria exhibited inhibition against the targeted bioassay oomycetes. Tentative identification of the bacteria was on the basis of 16S rRNA gene sequences which revealed 92-100% sequence identity to type strains of related species and placed these organisms in six major classes: Actinobacteria, γ-Proteobacteria, Firmicutes, α-Proteobacteria, Flavobacteriia and [beta]-Proteobacteria. When checked for lytic enzyme production, mostly the isolates of Actinobacteria and Firmicutes were potential enzyme producers. Detection of secondary metabolite biosynthetic genes - type I polyketide synthases, type II polyketide synthases and nonribosomal peptide synthetases - confirmed that 21 (35.5%) isolates were positive for at least one type of the biosynthetic gene. In order to identify metabolites, three isolates, Alteromonas australica (EA73), Aidingimonas halophila (EA105) and Halomonas zincidurans (EA127), were selected and subjected to chemical analyses using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Both analyses showed the presence of different bioactive compounds in the culture extracts of isolates some of which are already reported for their diverse biological activities such as 2, 4-Diacetylphloroglucinol. Our results demonstrated that halophytes represent an important source of potentially active bacteria producing antifungal metabolites of medical significance.en_US
dc.description.sponsorshipNational Plan for Science, Technology and Innovation (MAARIFAH)-King Abdulaziz City for Science and Technology; the Kingdom of Saudi Arabia (award number 12-BIO-2724-03);en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).en_US
dc.titleHalophytes-associated endophytic and rhizospheric bacteria: diversity, antagonism and metabolite productionen_US
mus.citation.journaltitleBiocontrol Science and Technologyen_US
mus.identifier.categoryLife Sciences & Earth Sciencesen_US
mus.relation.collegeCollege of Agricultureen_US
mus.relation.departmentPlant Sciences & Plant Pathology.en_US
mus.relation.universityMontana State University - Bozemanen_US

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